His Tag ELISA Detection Kit-L00436
2025-07-18
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Note
L00436 interferes with IgG in mouse/rat serum and is not suitable for detecting mouse/rat serum samples.
產(chǎn)品概述
Product Description
GenScript His Tag ELISA Detection Kit, a 1.5 hour competition ELISA, is developed for rapid and high throughput detection of His-tagged proteins. There are several potential usages:
●Quickly identify the presence of His-tagged proteins in samples.
●Optimize protein expression by monitoring the His-tagged proteins level.
●High throughput screening of stable cell lines expressing His-tagged proteins.
產(chǎn)品簡(jiǎn)介
Detection Range :1 ng/ml~729 ng/ml
Sensitivity: 1 ng/ml His-tagged proteins
Test Samples: N-terminal/C-terminal/internal His-tagged proteins4 x His/5 x His/6 x His-tagged proteins
Mammalian, yeast and bacteria cell lysates or cell supernatant
Conveniency :Provide all reagents required for test
Complete test within 1.5 hours
Reagents compatibility: Tolerable with various reagents at certain concentration
Storage :The unopened kit is stable for at least 12 months if stored at 2-8 °C and the opened kit may be stable for up to 1 month at 2-8 °C. Do not freeze the kit.
Kit Contents:
Component | Quantity | Part. No |
His Tag Plate | 1 plate (8 wells × 12 strips) | 436-80 |
Anti-His Monoclonal Antibody | 6 ml | 436-20 |
Antibody Tracer | 12 ml | 436-30 |
His Tag Standards (0,1,3,9,27,81,243,729 ng/ml) | 1 ml per each standard | 436-11,12,13,14,15,16,17,18 |
Assay Diluent | 60 ml | 436-60 |
20 × Wash Solution | 40 ml | B-A1-70 |
TMB Solution | 12 ml | B-A1-40 |
Stop Solution | 6 ml | B-A1-50 |
His Tag Standard Stock (10μg/ml) | 500 μl | 436-10 |
Plate Sealer | 2 | N/A |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
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