Catalog #

Product Description

ICP0380

This pan-specific antibody is affinity purified using acetyl-lysine affinity chromatography. It recognizes proteins with acetylated lysine residues. The product has been utilized for proteomic studies of acetylated proteins and immunoaffinity chromatography separation and isolation of acetylated proteins and peptides from protease-digesting proteins of whole cells.

A           B

Western blot analysis of the acetylated protein profile in HeLa cell lysate with anti-acetyl lysine antibodies (ICP0380, lane A) and with additional acetylated BSA (10 ug/mL) in the primary antibody (lane B).

Species

Rabbit

Formulation

250 μg/mL antibody stored in PBS, 50% glycerol

Immunogen

Acetylated KLH conjugates

Purification

The antibody was purified on acetyl-lysine agarose.

Specificity

The antibody recognizes proteins acetylated on lysine residues. Tested: acetylated histone, acetylated BSA, and acetylated MBP. There are no reactions to non-acetylated proteins.

Applications

ELISA; WB (1:1000); IP; Immunofluorescence;  Immunochemistry

Scientific Description

DNA transcription cannot take place unless DNA is unwound from the nucleosomes. The cell unwinds the DNA by acetylating lysine residues within the proteins. It has been suggested that acetylation of non-histone proteins (e.g., transcription factors) and histones may be involved. Acetylation of these proteins may result in signal transduction within the chromatic domains.

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is three years from date of shipping if properly stored.

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