CCL-46 P388D1 小鼠淋巴樣瘤細(xì)胞
ATCC® Number:  CCL-46™      
Designations:  P388D1 
Depositors:   EM Jensen 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: suspension
Organism: Mus musculus （mouse） 
Morphology: lymphoblast

 
Source: Disease: lymphoma
Strain: DBA/2
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
 
Applications: Biological response [92560]
transfection host （Roche FuGENE® Transfection Reagents）
Tumorigenic: Yes 
Reverse Transcript: positive 
Antigen Expression: H-2, d haplotype; Mus musculus, expressed
Comments: A subclone of this line [see ATCC TIB-63, P388D1（IL-1）] produces high levels of interleukin-1 （IL-1, interleukin 1）.Tested and found negative for ectromelia virus （mousepox）.
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide （CO2）, 5%
Temperature: 37.0°C
Subculturing:  Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 - 5 X 10 （5） viable cells/ml.
A maximum of 6 X 10 （6） viable cells/ml is obtainable.
Medium Renewal: Every 2 to 3 days
Preservation:  Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time:  10 to 12 hrs 
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002
recommended serum:ATCC 30-2040
Cell culture tested DMSO:ATCC 4-X
References: 22208: . . Am. J. Pathol. 33: 603, 1957.
92560: Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 1903-98R03.