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轉(zhuǎn)染試劑

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轉(zhuǎn)染試劑

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起發(fā)實驗試劑有限公司是專業(yè)從事生物技術(shù)產(chǎn)品銷售的高科技企業(yè)，是國內(nèi)生物試劑、儀器、消耗品領(lǐng)域的企業(yè)之一。起發(fā)公司自成立以來，連續(xù)2年高速成長。公司的發(fā)展目標是建成專業(yè)從事生物技術(shù)產(chǎn)品銷售、生產(chǎn)與開發(fā)的*公司。

起發(fā)實驗試劑有限公司已成為cygnus , TriLink BioTechnologies ,crystal chem., SYnaptic Systems,polysciences，research diets在華的代理或*代理。



起發(fā)實驗試劑有限公司在香港和北京和上海設(shè)有分公司或辦事處。銷售的產(chǎn)品涵蓋生物技術(shù)領(lǐng)域的各個方面：分子生物學(xué)試劑、生物化學(xué)試劑、免疫學(xué)試劑、科研儀器、臨床診斷與檢驗儀器以及其它科研用消耗材料等。  

emsdiasum,AVANTI,Angiobio,Cedarlane,Cisbio,trc-canada,cygnus,covance(crp),vectorlabs,tedpella

 origene

多種轉(zhuǎn)染試劑滿足您的特定的cDNA和shRNA轉(zhuǎn)染需求。

Cell Types Examples Recommended Reagents
Commonly used established cell lines Hela, MCF 7, K562, Jurkat, 3T3, COS TurboFectin 8.0
Most established cell lines and primary cells Above, plus MEF, HUVEC, SMC, lymphocytes, etc. Magnetofection PolyMag
Magnetofection CombiMag
Primary neurons and neuronal cell lines Hippocampal neurons and neuronal cell lines PC-12, B95, C6, N2A or SH-5YSY, etc. Magnetofection NeuroMag
Virus infection of non-permissive cells   Magnetofection ViroMag
HEK293T, CHO, 293S, etc   MegaTran 1.0
For siRNA transfection HEK293T siTran 1.0
  • TurboFectin 8.0TM是針對核酸轉(zhuǎn)染真核細胞所優(yōu)化的一種高效率的新型轉(zhuǎn)染試劑，由配方的脂質(zhì)體和組蛋白混合物，溶在80%的酒精中。適用于在各種不同構(gòu)建類型的細胞中轉(zhuǎn)染TrueClone®/TrueORF®克隆 （基因過表達） 以及 HuSH-29® （用于基因沉默的shRNA）。
    • 性能*: 與FuGENE®6 相媲美的高轉(zhuǎn)染效率和低毒性
    • 應(yīng)用廣泛: 使用TurboFectin 8.0 已經(jīng)成功轉(zhuǎn)染超過100種細胞系及原代細胞類型
    • *: 比FuGENE®價格便宜
  • Magnetofection TM是一種新型, 簡易，高效的轉(zhuǎn)染方法。利用磁力將含有磁珠的目的基因載體，吸向靶細胞。
    • 可用于更多種細胞，更加適用于難轉(zhuǎn)染的細胞。包括原代細胞和神經(jīng)細胞
    • 與常規(guī)轉(zhuǎn)染方法相比，轉(zhuǎn)染效率*提高。
    • 在較短的孵育過程內(nèi)，擁有比常規(guī)轉(zhuǎn)染方法高上千倍的轉(zhuǎn)染效率
    了解更多  春季*
     
  • MegaTran 1.0 是一種非脂質(zhì)體聚合物，適用于大量DNA和shRNA轉(zhuǎn)染及大規(guī)模蛋白質(zhì)制備。
    適用細胞系：CHO，HEK293，293F suspension cells，Hela，COS-7，NIH3T3，MEF，Sf9，U2OS等。
    • 高效，低毒。
    • 提高瞬時轉(zhuǎn)染后蛋白的表達量。
    • 價格非常優(yōu)惠。

INVITROGEN

 

Transfection Reagent Key Features & Applications of Reagent
Lipofectamine™ LTX Transfection Reagent        
 
  • High transfection efficiency and significantly lower toxicity levels for a wide range of cell lines
  • Significantly improved transfection performance in a number of primary and hard-to-transfect cell lines
  • Optimized protocols for over 30 different cell lines are available so much less time is needed for evaluation and optimization
FreeStyle™ MAX Transfection Reagent
 
  • Optimized for transient transfection in CHO suspension cells and also works for HEK-293 cells
  • Used for a large-scale transient protein production with milligrams of protein yield
Lipofectamine™ RNAiMAX Transfection Reagent
 
  • Superior transfection efficiency requiring lower RNAi concentrations leading to more effective gene knockdown with minimal non-specific effects
  • Easy optimization due to minimal cytotoxicity across a 10-fold concentration range of transfection reagent
  • Compatibility with a broad range of cell types providing the most versatile approach to all of your gene silencing experiments
Lipofectamine™ 2000 Transfection Reagent
 
  • Achieves highest efficiency and expression results for plasmid or RNAi transfections
  • Works effectively with many cell types （adherent or suspension）
  • Easy and fast protocol – no need for wash steps before or after transfection
  • Complexes can be added to cells growing in serum-containing media
  • Ideal if transfecting at confluencies of 90% or greater （minimizes cytotoxicity following transfection）
Lipofectamine™ 2000 CD Transfection Reagent
 
  •  Same performance as Lipofectamine™ 2000, certified animal-origin free （“CD” = chemically defined）
Oligofectamine™ Transfection Reagent 
 
  • Transfection of antisense oligonucleotides
293fectin™ Transfection Reagent
 
  • Used for transient protein production in combination with the FreeStyle™ 293 Expression System
  • Optimized for suspension FreeStyle™ 293-F cells
Cellfectin® Transfection Reagent
 
  • Optimal transfection of insect cells, including S2, Sf9, Sf21 and High Five™ cells
DMRIE-C Transfection Reagent
 
  • Transfection of suspension cells, including CHO, lymphoid and Jurkat cell lines.
Lipofectamine™ Transfection Reagent
 
  • First generation reagent for plasmid DNA transfections
  • In most cases, Lipofectamine™ 2000 provides better performance than Lipofectamine™ Transfection Reagent
Lipofectin® Transfection Reagent
 
  • First generation reagent for plasmid DNA transfections
  • In most cases, Lipofectamine™ 2000 provides better performance than Lipofectin® Transfection Reagent
Optifect™ Transfection Reagent
 
  • Broad use reagent designed for low confluency applications （<70% confluent at the time of transfection）
  • Useful for cell lines that are sensitive to transfection reagents

 

Plasmid DNA Transfection

To obtain efficient gene transfer by transfection, plasmid DNA can be complexed with lipid reagents to mediate efficient delivery into the cell's nucleus. This process is essential for subsequent protein expression of the gene of interest. Plasmids are small circular DNA molecules that naturally occur in bacteria, and are actually used by the bacteria to transfer genetic information. The mechanism of adding a DNA Plasmid into a mammalian cell is known as plasmid transfection.

Invitrogen offers a wide range of transfection reagents for your specific needs.  For cell types specific protocols, please refer toTransfection Selection Tool.

  • Order:
    • Plasmid Transfection Reagents
    • Electroporation Instruments & Reagents

Featured Product - Lipofectamine™ LTX with PLUS™ Reagent

Our latest and most efficient Lipofectamine™ product. The best choice for transfecting plasmid DNA into standard and other difficult to transfect cell types; highest transfection efficiency and viability with superb expression for all cell types. 

  • High transfection efficiency and high gene expression with minimal cytotoxicity in a broad range of cells
  • Significantly improved transfection performance in a number of primary and disease-relevant cell types
  • No washes or media changes required - just set up and go!

In 90% of the cell lines that we have tested, transfection performance is enhanced by using Lipofectamine™ LTX and PLUS™ Reagent together.

  • Learn more about Lipofectamine™ LTX with PLUS™ Reagent

Ordering Information

 

 

Sku Product Catalog Size Price Quantity
15338-100 Lipofectamine™ LTX & Plus Reagent 1 ml
 		 488.43 USD
 		  
15338-500 Lipofectamine™ LTX Reagent 15 ml
 		 6592.80 USD
 

 

 



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