alchemyTM HPLC Columns
The Queen of Beauty in Chromatography




A Major Advance in HPLC Columns through Nanotechnology

alchemyTM HPLC columns are made of spherical silica particles manufactured of the highest purity (〉99.999%) in the market. The highest quality silica, an innovative surface nano-modification and unique bonding processes result in exceptional C18 phases with outstanding properties. One of the most remarkable properties of every alchemyTM column is that all types of organic compounds (neutral, basic or acidic) will have perfect peak symmetry and excellent efficiency across the whole applicable pH range. The proprietary nano-surface modification generates a very smooth and non-reactive surface, reducing the interaction between the silica surface and polar compounds, especially the basic compounds. Lot-to-lot reproducibility is ensured by a stable bonding/end-capping process that is extremely rugged. The uniform, spherical particles have a nominal surface area of 360 m2/g with a controlled pore size of 100Å. All columns are packed using a consistent slurry packing process to achieve uniform and stable bed for maximum column efficiency, lifetime and column-to-column reproducibility.
Unprecedented Experience in Chromatography

With the alchemyTM columns, you will have an unprecedented experience in HPLC separation.
Best peak symmetry and efficiency. This is one of the most remarkable features of our columns. They just outperform competitors’ products across the whole applicable pH range. Their peak shape and column efficiency are independent of pH. This provides maximum flexibility for method development and optimum ruggedness for the methods developed.

Tightest specification. All our columns have tighter specification (see Table 2) than those of brand name manufacturers. This ensures that each column has great performance and identical se-lectivity.



Best value. Because of our unique business structure and well-controlled processes, we are able to provide
products of the highest quality at very competitive price; we are also able to customize any of our products based on customer requirements.
Broad se-lection on prices and features. We offer a full line of products for a wide variety of applications and are willing to assist customers in optimizing their particular applications




特征of alchemyTM columnsthe columns’用戶效果
Perfect peak symmetry, independent of pH and phase Highest flexibility for any application,Better sensitivity,Greater confidence on HPLC results
Broad pH range (1-10.5)Flexibility for method development
Compatible with * aqueous phaseMaximum versatility
Highest surface coverageTolerance of large volume and mass injection
A wide variety of chemistriesBroad se-lection of polarity and Maximum versatility
Minimum buffer concentration neededBetter sensitivity for LC/MS, extended column lifetime
Tightest specificationBetter reproducibility, Greater confidence on the HPLC results
Excellent column efficiencyBetter and faster separation
Great column lifetimeLower cost; greater convenience

Comparison of features and specification

Waters Symmetry-C184.6 x 150 mm, 5umZorbax XDB-C184.6 x 150 mm, 5um alchemy XBP-C184.6 x 150 mm, 5um
Spec. on efficiencyna800011000
Spec. on tailing factor (toluene) na0.98-1.250.96-1.10
Spec. on tailing factor-amitriptyline1.45na (typically 1.5-2.0)1.25
Spec. on se-lectivity(amitriptyline/acenaphthene)+/- 10%na+/- 7%
pH range2-82-91.5-10.5
Specific Surface 300180360
Carbon%16%12%22%

Inertness and efficiency comparison



Sample: uracil, toluene, doxepin , amitriptyline：
Column: 4.6 x 150, 5 um
phase: 10 mM sodium phosphate (pH=7) in 60% ACN.
Temperature: 30 oC;
Flow（: 1 mL/min.
The plate numbers in regular font are for toluene, the plate numbers in bold are for amitriptyline
Volume Loading Capacity Comparison


Large Volume Injection (injection volume =100 uL, ) Sample（: 4-methoxybenzenesulfonamide, 10 ug/mL in MeOH/Water (1:1)
色譜柱: 4.6 x 150 mm, 5um particle size
phase: MeOH/Water
Temperature: 30 oC
Flow: 1mL/min.


Mass Load Comparison






















Columns（: 21.2 x 150 mm, C18, 5um
Sample: diphenhydramine, 100 mg/mL in DMSO,
800uL
phase: 65% 10 mM ammonia: 35% ACN





A Wide Selection of alchemy TM Columns

To meet differing application needs with-in the corresponding pH requirements, we have the following offerings available in our reverse phase line-up:

alchemyTM XBP series, XBP-C18 and XBP-C8, are bonded to the maximum density to have the highest hydr-ophobicity (the lowest polarity), the lowest interaction of analytes with silanols, high stability at high pH and the broadest pH range. alchemyTM XBP系列，XBP-C18和XBP-C8，

alchemyTM AQ-C18 columns are more polar than XBP and have more separation power for polar compounds. Due to special silica treatment, they are true C18 columns that are compatible with * aqueous phase. Like XBP columns, they are still very friendly towards those very basic analytes. Although their upper pH limit is not as high as XBP, it is comparable to many popular brand name columns.

alchemyTM LP-C18 columns are the most polar among C18 columns due to a polar end-cap and are preferred replacement for Waters Atlantis and Zorbax SB-C18. They are extremely stable at low pH and may also be used at mid-pH. Unlike Atlantis and SB-C18, LP-C18 columns can still maintain perfect peak symmetry even for very basic analytes and are compatible with * aqueous phases.在 C18 columns中alchemyTM LP-C18 columns

Comparison of alchemyTM -C18 Phases
XBP-C18AQ-C18LP-C18
Surface area (m2/g)360360360
C%22%18%17%
End-capdoublesinglepolar
PolarityIncrease à
pH stability1.5-10.51.5-91-7
* water compatible noyesyes
Peak shape of bases excellentexcellentexcellent
ReplacedcolumnsZobax XDBSymmetryXterraLunaDiscoverySymmetryLuna (2)Inertsil ODS-3AQ phasesZorbax SB-C18Zorbax SB-AQWaters AtlantisAQ phases
Use of organic base for pH 〉 9 is recommended .







Test conditions:
columns:2.1 x 50 mm, 5um;
phase: 60% or 65% MeOH in water;
flow:0.2 mL/min
temperature: 30 oC
injection:1uL
sample:uracil, phenol, chloronitrobenzene (CNB), toluene (TOL)
detection:UV 254 nm



























alchemy XBP Columns


alchemyTM XBP columns, XBP-C18 and XBP-C8, are bonded to the maximum density to have the highest hydr-ophobicity (the lowest polarity), the lowest interaction of analytes with silanol, high stability at high pH and the broadest pH range.
Pore size: 100 A; Specific surface: 360 m2/g; Purity 〉 99.999%;

1)Inertness: one of most base-friendly columns.
2)Efficiency: the best for basic compounds at any applicable pH.
3)Large volume injection: maintain very high efficiency even if the injection volume is exceptional large.
4)Broad pH range (1.5 – 10.5 ): Low pH, 0.2 % TFA; High pH, 0.010 M triethylamine.


Column packing quality



Sample: uracil, phenol, chloronitrobenzene, toluene
(in methanol/water=50/50)
Columns: 5 um, 2.1 x 50 mm
phase: methanol/water (65/35)
Temperature: 30 oC
Flow: 0.20 mL/min

Plates (toluene)Tailing Factor
Zorbax XDB-C836051.18
Luna C825461.15
Venusil XBP-C8 40121.03






Great stability at low pH



Column: Venusil XBP –C18, 4.6 x 150 mm, 5 um
Aging: 40 oC, pH= 1.0, TFA in 80 % Methanol, 400 hours,
Flow= 1.0 mL/min
Testing: 30 oC, pH=1.5, TFA in 80% methanol, 5 uL inj. of
naphthlene


Perfect peak symmetry and great lifetime of the column for basic compounds at mid-pH



Sample: Uracil, Doxepin, Nortriptyline, Amitriptyline,
Trimipramine
Column: Venusil XBP-C18, 4.6 x 150 mm,
phase: 0.01 M sodium phosphate/ACN (50/50),
pH=7.6
Flow: 1.5 mL/min
Temperature: 45oC








Ordering Information

Analytical alchemyXBP-C18 Columns
Particle Size 5µm2.1mm ID cat.#4.6mm IDcat.#
30mm LengthVX950302-0VX950305-0
245.00260.00
50mm LengthVX950502-0VX950505-0
245.00270.00
100mm LengthVX951002-0VX951005-0
295.00320.00
150mm LengthVX951502-0VX951505-0
335.00360.00
250mm LengthVX952502-0VX952505-0
inquire375.00

Particle Size 3µm2.1mm ID cat.#4.6mm ID cat.#
30mm LengthVX930302-0VX930305-0
260.00285.00
50mm LengthVX930502-0VX930505-0
260.00315.00
100mm LengthVX931002-0VX931005-0
295.00375.00
150mm LengthVX931502-0VX931505-0
355.00389.00


Analyticalalchemy XBP-C8 Columns
Particle Size 5µm2.1mm ID cat.#4.6mm ID cat.#
30mm LengthVX850302-0VX850305-0
245.00260.00
50mm LengthVX850502-0VX850505-0
245.00270.00
100mm LengthVX851002-0VX851005-0
295.00320.00
150mm LengthVX851502-0VX851505-0
335.00360.00
250mm LengthVX852502-0VX852505-0
inquire375.00





Preparative alchemy XBP-C18 Columns
Particle Size 10µm20mm ID cat.#30mm ID cat.#
50mm LengthVX900520-0VX900530-0
795.00inquire
100mm LengthVX901020-0VX901030-0
980.002200.00
150mm LengthVX901520-0VX901530-0
1180.002850.00
250mm LengthVX902520-0VX902530-0
1590.003490.00

Particle Size 5µm20mm ID cat.#30mm ID cat.#
50mm LengthVX950520-0VX950530-0
895.00inquire
100mm LengthVX951020-0VX951030-0
995.002290.00
150mm LengthVX951520-0VX951530-0
1380.002950.00
250mm LengthVX952520-0VX952530-0
1890.003890.00














alchemy AQ C18 Columns


alchemyTM AQ-C18 columns are designed for separation of polar and semi-polar compounds at low to medium pH. They are more polar than XBP-C18 and less polar than LP-C18. They are true C18 columns. However, due to a special surface treatment , they are compatible with * aqueous phase. They are stable at low pH 1.5 – 9.0 and are preferred replacement for Waters Symmetry, Inertsil C18, Zorbax SB, and many AQ columns.
Pore size: 100 A; Specific surface: 360 m2/g; Purity 〉 99.999%;
1)Inertness: One of most base-friendly columns, excellent peak symmetry of basic compounds.
1)Efficiency: best for polar compounds at any pH.
1)Large volume injection: Maintains very high efficiency even if the injection volume is exceptional large.
1)* water compatible: Very unique for a universal C18 column; far better peak shape, retention and efficiency than those so-called high aqueous “AQ” columns.
1)Low pH Stability ( pH=1.5 ): Better stability than most of popular brand columns on the market and much more stable than most of AQ columns .

Perfect peak symmetry and good stability at med-pH for basic compounds



Sample: Uracil, Doxepin, Nortriptyline, Amitriptyline,
Trimipramine
Column: alchemy AQ-C18, 4.6 x 250 mm,
phase: 0.01 M sodium phosphate/ACN (25/75),
pH=7.0
Flow: 1.3 mL/min. Temperature: 30oC


Compatibility with * aqueous phase


Sample: Uridine
Column: 4.6 x 150 mm
phase: * water
Flow: 1mL/min.; the flow was stopped for 5 minutes during
the testing period for each column.
Temperature: 30 oC

Stability of alchemy AQ-C18 at pH=1.5


Sample: Uracil, Doxepin, Amitriptyline, Toluene.
Column: alchemy AQ C18, 4.6 x 150 mm, 5 um
phase: TFA (0.25%, pH=1.5) in 60% acetonitrile
Flow: 1 mL/ min.;
Temp: 40 oC




Ordering Information


Analytical alchemyAQ-C18 Columns

Particle Size 5µm2.1mm ID cat.#4.6mm ID cat.#
30mm LengthVA950302-0VA950305-0
245.00260.00
50 mm LengthVA950502-0VA950505-0
270.00285.00
100mm LengthVA951002-0VA951005-0
295.00320.00
150mm LengthVA951502-0VA951505-0
335.00370.00
250mm LengthVA952502-0VA952505-0
inquire390.00


Particle Size 3µm2.1mm ID cat.#4.6mm ID cat.#
30mm LengthVA930302-0VA930305-0
260.00285.00
50mm LengthVA930502-0VA930505-0
260.00315.00
100mm LengthVA931002-0VA931005-0
295.00375.00
150mm LengthVA931502-0VA931505-0
355.00389.00




















Preparative alchemy AQ-C18 Columns

Particle Size 10µm20mm ID cat.#30mm ID cat.#
50mm LengthVA900520-0VA900530-0
920.001980.00
100mm LengthVA901020-0VA901030-0
1090.002200.00
150mm LengthVA901520-0VA901530-0
1190.002500.00
250mm LengthVA902520-0VA902530-0
1590.003890.00





















alchemy LP Columns



alchemy LP columns are specially designed for separation of polar compounds at very low to medium pH. They are end-capped with polar groups and have the highest polarity among Venusil columns. They are extremely stable at low pH (pH= 1) and are preferred replacement for Waters Atlantis, Zorbax SB, and many AQ columns.

Pore size: 100 A; Specific surface: 360 m2/g; Purity 〉 99.999%;
1Low pH Stability ( pH=1): it has the best stability at low pH
2Peak shape and efficiency: Unlike many polar phase columns, it has excellent peak symmetry of basic compounds.
3Polar C18 phase very strong separating power for polar compounds
4Large volume injection: maintain very high efficiency even if the injection volume is exceptional large.
5* water compatible: better peak shape, retention and efficiency than those so-called high aqueous “AQ” columns.


Ordering Information


Analytical alchemy LP-C18 Columns
Particle Size 5µm2.1mm IDCat. # 4.6mm ID Cat. #
30mm LengthVL950302-0VL950305-0
245.00260.00
50mm LengthVL950502-0VL950505-0
270.00285.00
100mm LengthVL951002-0VL951005-0
295.00320.00
150mm LengthVL951502-0VL951505-0
335.00370.00
250mm LengthVL952502-0VL952505-0
355.00390.00







Particle Size 3µm2.1mm IDCat. # 4.6mm ID Cat. #
30mm LengthVL930302-0VL930305-0
260.00285.00
50mm LengthVL930502-0VL930505-0
260.00315.00
100mm LengthVL931002-0VL931005-0
295.00375.00
150mm LengthVL931502-0VL931505-0
355.00389.00