化工儀器網(wǎng)>產(chǎn)品展廳>試劑標(biāo)物>行業(yè)專用試劑>生化與分子生物學(xué)用試劑>貨號：AS04 054- plant alternative oxidase antibody
貨號：AS04 054- plant alternative oxidase antibody
| 參考價 | ￥ 3975 |
| 訂貨量 | ≥1 |
- 公司名稱 上海經(jīng)科化學(xué)科技有限公司
- 品牌 Agrisera
- 型號 貨號：AS04 054-
- 產(chǎn)地
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- 更新時間 2016/5/20 16:31:44
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AOX1/2|plant alternative oxidase antibody介紹：
貨 號：AS04 054
中文名稱：兔抗植物交替氧化酶1&2多克隆抗體
英文名稱：AOX1/2|plant alternative oxidase 1 and 2
應(yīng)用：western blot (WB)
規(guī)格：200 µl
價格：3975元
相關(guān)抗體：
貨號：AS06 152---alternative oxidase 1抗體
貨號：AS04 054---plant alternative oxidase antibody
AOX1/2|plant alternative oxidase antibody簡介：
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| APPLICATION INFORMATION | ||
| recommended dilution | 1 : 1000 on 10-20 µg of mitochondrial protein/lane with standard ECL detection (WB), 1: 750 (IL) | |
| expected | apparent MW | 36-40 | 36-40 for Arabidopsis thaliana | |
| confirmed reactivity | Arabidopsis thaliana, Betula nana, Beta vulgaris, Brassica napus, Kandelia candel, Eriphorum vaginatum, Hordeum vulgare, Lupinus luteus, Nicotiana tabacum, Picea abies, Pisum sativum,Robinia pseudoacacia, Solanum tuberosum, Solanum esculentum, Physcomitrella patens | |
| predicted reactivity | Aegilops tauschii, Brachypodium distachyon, Capsella rubella, Citrus sinensis, Citrus clementina, Corylus heterophylla, Crocus sativus, Cucumis sativus, Daucus carota, Glycine max, Hypericum perforatum, Lotus japonicus, Malus x domestica, Medicago truncatula, Medicago sativa, Nicotiana benthamiana, Oryza brachyantha, Oryza sativa, Populus tremula, Picea sitchensis, Triticum aestivum, Saccharum officinarum, S.officinarumea, Sorghum bicolor, Selaginella moellendorffii, Tetrahymena thermophila, Zea mays, Vigna unguiculata, Vitis vinifera | |
| not reactive in | Chlamydomonas reinhardtii | |
| additional information | According to Konert et al. (2015) AOX antibody is recognizing AOX1A and AOX1D. | |
| selected references | Meng et al. (2016). Physiological and proteomic responses to salt stress in chloroplasts of diploid and tetraploid black locust (Robinia pseudoacacia L.). Sci Rep. 2016 Mar 15;6:23098. doi: 10.1038/srep23098 Pavlovi? et al. (2016). Light-induced gradual activation of photosystem II in dark-grown Norway spruce seedlings. Biochim Biophys Acta. 2016 Feb 18. pii: S0005-2728(16)30028-7. doi: 10.1016/j.bbabio.2016.02.009. Konert et al.(2015). Protein phosphatase 2A (PP2A) regulatory subunit B'γ interacts with cytoplasmic ACONITASE 3 and modulates the abundance of AOX1A and AOX1D in Arabidopsis thaliana. New Phytol. 2015 Feb;205(3):1250-63. doi: 10.1111/nph.13097. Epub 2014 Oct 13. Wang et al. (2015). Proteomic analysis of changes in the Kandelia candel chloroplast proteins reveals pathways associated with salt tolerance. Plant Sci. 2015 Feb;231:159-72. doi: 10.1016/j.plantsci.2014.11.013. Epub 2014 Dec 4.? Dimkovikj and Van Hoewyk (2014). Selenite activates the alternative oxidase pathway and alters primary metabolism in Brassica napus roots: evidence of a mitochondrial stress response. BMC Plant Biol. 2014 Sep 30;14:259. doi: 10.1186/s12870-014-0259-6. Tsaniklidis et al. (2014). Study of alternative oxidase in seeded and parthenocarpic cherry tomato fruits during their development and postharvest storage. Acta Physiologiae Plantarum, September 2014. (immunolocalization) Zubo et al. (2014). Inhibition of the electron transport strongly affects transcription and transcript levels in Arabidopsis mitochondria. Mitochondrion. 2014 Mar 31. pii: S1567-7249(14)00037-3. doi: 10.1016/j.mito.2014.03.011 | |
application example 25 μg of Arabidopsis thaliana mitochondrial wild type fraction (1) mitochondrial fraction from a mutant with increased AOX level (2), total wild type leaf extract (3), total leaf extract from AOX overproducing mutant (4) were separated on 10% gel and blotted on nitrocellulose membrane using wet transfer (0.22% CAPS, pH 11). Filters where blocked (1.5h) in 5% milk in TBST (1X TBS, 0,1% Tween 20), incubated with 1: 1000 anti-AOX polyclonal antibodies (2h in TBST) followed by 1 h incubation with 1: 50 000 Agrisera secondary anti-rabbit HRP-coupled antibodies (AS09 602) and visualized with standard ECL on Kodak autoradiography film for 15-60 s. Mitochondria were isolated as described by Urantowka et al. (Plant Mol Biol, 2005, 59:239-52). Mitochondrial pellets were suspended in 1X Laemmli buffer (5% beta-mercaptoetanol, 3.7% glycerol, 1.1% SDS, 23 mM Tris- HCl pH 6.8, 0.01% bromophenol blue), heated (95°C, 5 min.) and centrifuged (13 000rpm, 1 min.). Leaf extracts were prepared as described by Martinez-Garcia et al. (Plant J., 1999, 20:251-7).
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AOX1/2|plant alternative oxidase antibody
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