Product Description

Affinity purified bovine IgG antibodies are developed from rabbit using affinity purified bovine IgG as the immunogen. The bovine antibody-specific antibodies from the rabbit immune serum are affinity purified using pure bovine IgG as the affinity matrix. The purified rabbit antibodies are useful for detection, identification, quantization and localization of bovine antibodies (IgG's).

Species

Rabbit

Formulation

1 mg/mL in PBS with 50% glycerol

Immunogen

Affinity purified bovine immunoglobulin G (whole molecule)

Purification

The antibody is affinity purified with bovine antibodies immobilized on agarose and absorbed with IgG from human, rabbit and bovine.

HRP/Ab Ratio 

2:1

Specificity

The antibody is specific against bovine IgG. There are no cross reactions with human or rabbit Ig's.

Applications

ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL); IHC

Scientific Description

IgG is monomeric immunoglobulin. It is built of two heavy chains and two light chains. Each IgG has two antigen binding sites. It is the most abundant immunoglobulin in the blood and tissue. It provides protection against many kinds of bacteria, viruses, fungi as well as optimizes phagocytosis and neutralizes bacterial toxins.

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is three years from date of shipping if properly stored.

References

1. Junqueira, L. C. et al. Basic Histology, 2003 McGraw-Hill.