Culture Patch片上器官培養(yǎng)貼片

文化補(bǔ)丁

培養(yǎng)貼片包括沉積在支撐微框架上的納米纖維單層。該培養(yǎng)貼片可用于三維細(xì)胞培養(yǎng)和組織形成。它允許細(xì)胞在復(fù)雜的3D組織中的自組裝，使外源性接觸小化，并大化細(xì)胞攝取。

納米纖維可以由不同的天然和合成材料制成：

明膠，

膠原蛋白，

聚乳酸-乙醇酸，

聚己內(nèi)酯，

聚二甲基戊二酰亞胺

納米纖維的取向可以是：

隨機(jī)的

對齊的

支撐納米纖維的蜂窩微框架可以制成不同的尺寸、形狀和彈性您可以在兩種建筑材料之間進(jìn)行選擇：

聚乙二醇二丙烯酸酯（1.2兆帕的年輕模塊）

聚二甲基硅氧烷

為了培養(yǎng)你自己的三維細(xì)胞培養(yǎng)，貼片可以簡單地放在培養(yǎng)皿、多孔、博伊登室中，或者直接放在兼容的微流控芯片中（單擊此處獲取有關(guān)芯片上器官設(shè)置的更多信息）在實(shí)驗(yàn)結(jié)束時，可以從設(shè)備上移除貼片以進(jìn)行進(jìn)一步分析（如成像、蛋白質(zhì)組學(xué)和基因組學(xué)）。

Culture Patch片上器官培養(yǎng)貼片

Culture Patch

A culture patch consists in a monolayer of nanofibers deposited on a supporting micro-frame. This culture patch can be used for 3D cell culture and tissue formation. It allows the self-assembly of cells in complex 3D tissues minimizing the exogenous contact and maximizing the cellular uptake.

Nanofibers can be made of different natural and synthetic materials:

• gelatin,
• collagen,
• PLGA poly(lactic-co-glycolic acid), 
• PCL polycaprolactone,
• PMGI polydimethylglutarimide

The orientation of nanofibers can be:

• Random
• Aligned

Honeycomb micro-frames that support the nanofibers can be made with different size, shape and elasticity. You can choose between two building materials:

• PEGDA poly(ethylene glycol)diacrylate (Young module of 1.2 MPa)
• PDMS polydimethylsiloxane (Young module of 0.5 MPa)

To grow your own 3D cell culture, the patch can be simply placed in a Petri-dish, multi-wells, Boyden chambers or directly in a compatible microfluidic chip (click here for more information on the Organ-on-Chip setup). At the end of your experiment, the patch can be removed from the device for further analyses (e.g. imaging, proteomics and genomics).