SCC-4人舌鱗癌細(xì)胞（附ST
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人舌鱗癌細(xì)胞SCC-4
Soil enzyme activity: Using 10 % urea as the substrate, the 24-h soil urease activity was measured based on Tabatabai and Bremner's method. The soil enzyme activities of NIT and AMO were determined using ELISA kits from Shanghai Fuyu
Biotechnology Co., Ltd., (Shanghai, China), and that of NR was determined using the Beijing Solarbio NR Assay Kit (Beijing, China).
| 種屬 | 人 |
| 別稱 | SCC 4; SCC4 |
| 組織來(lái)源 | 來(lái)源于取樣部位：口腔；舌頭 |
| 疾病 | 鱗狀細(xì)胞癌 |
| 傳代比例/細(xì)胞消化 | 1:2傳代 ，消化2-3分鐘 |
| 培養(yǎng)基配置 | DMEM培養(yǎng)基；10%胎牛血清；1%雙抗 |
| 簡(jiǎn)介 | SCC-4 從一名 55 歲男性鱗狀細(xì)胞癌患者的舌頭中分離出來(lái) |
| 形態(tài) | 上皮細(xì)胞樣 |
| 生長(zhǎng)特征 | 貼壁生長(zhǎng) |
| 倍增時(shí)間 | 每周 2 至 3 次 |
| 基因表達(dá) | Yes, Tumors developed within 21 days at 99% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. |
| 致瘤性 | epidermal keratins; 40 kD keratin |
| STR | Amelogenin: X,Y CSF1PO: 11 D13S317: 11,13 D16S539: 12 D5S818: 13 D7S820: 9,11 THO1: 9.3 TPOX: 8 vWA: 15,17 |
| 培養(yǎng)條件 | 氣相：空氣 ，95% ；二氧化碳 ，5%。 溫度：37攝氏度 ，培養(yǎng)箱濕度為70%-80%。 |
| 凍存條件 | 凍存液：90%FBS ，DMSO 10%, 或使用非程序凍存液：貨號(hào)JY-H040 |
| 保藏機(jī)構(gòu) | ATCC; CRL-1624 |
| 產(chǎn)品使用 | 于科學(xué)研究 ，不可作為動(dòng)物或人類疾病的治療產(chǎn)品使用。 |
Soil enzyme activity: Using 10 % urea as the substrate, the 24-h soil urease activity was measured based on Tabatabai and Bremner's method. The soil enzyme activities of NIT and AMO were determined using ELISA kits from Shanghai Fuyu
Biotechnology Co., Ltd., (Shanghai, China), and that of NR was determined using the Beijing Solarbio NR Assay Kit (Beijing, China).
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