SK-MEL-5人黑色素瘤細胞
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- 公司名稱 上海富雨生物科技有限公司
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- 更新時間 2025/4/24 11:38:26
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皮膚惡性黑色素瘤細胞SK-MEL-5
19156044901 （同微信）
the mRNA levels of TGF- β1 and TIMP－2，and the protein levels of TGF- β1，TIMP－2，
and p- β-catenin / β-catenin were obviously increased (P＜0．05) ，the corresponding index of TAK－715 group showed the opposite trends ( P＜0．05) ;
compared with the ATR group，the axial length，refraction，the levels of IL－1β , IL－6 and TNF- α , the mRNA level of MMP－2，
and the protein lev- els of Wnt and MMP－2 in the ATR+TAK－715 group were obviously
| 種屬 | 人 |
| 別稱 | SK-Mel-5; SK MEL 5; SK.MEL.5; SK-MEL5; SKMel-5; SKMEL-5; SKMEL5; SKMel5; SKmel5; AA-Mel |
| 組織來源 | 皮膚 |
| 疾病 | 皮膚黑色素瘤 |
| 傳代比例/細胞消化 | 1:2傳代,消化2-3分鐘 |
| 培養(yǎng)基配置 | MEM培養(yǎng)基；10%胎牛血清；1%雙抗 |
| 簡介 | SK-MEL-5 is a melanoma cell line isolated from skin tissue obtained from a 24-year-old, White, female malignant melanoma patient. This cell line is a suitable transfection host and also serves as a source of target cells for the detection of melanoma-specific antibody in patients with the disease. This is one of a very extensive series of melanoma lines isolated by T. Takahashi and associates. |
| 形態(tài) | 星狀細胞樣 |
| 生長特征 | 貼壁生長 |
| 倍增時間 | 每周 2 至 3 次 |
| 抗原表達 | Blood Type O; Rh +; HLA A2, A11, B40, Bw16 |
| 致瘤性 | Yes, in nude mice; forms malignant melanoma |
| STR | Amelogenin: X CSF1PO: 10,13 D13S317: 10,12 D16S539: 10,12 D5S818: 11,13 D7S820: 9,12 THO1: 6,9 TPOX: 11 vWA: 14,18 |
| 培養(yǎng)條件 | 氣相：空氣 ，95% ；二氧化碳 ，5%。 溫度：37攝氏度 ，培養(yǎng)箱濕度為70%-80%。 |
| 凍存條件 | 凍存液：90%FBS ，DMSO 10%, 或使用非程序凍存液：貨號JY-H040 |
| 保藏機構(gòu) | ATCC; HTB-70 |
| 產(chǎn)品使用 | 于科學(xué)研究 ，不可作為動物或人類疾病的治療產(chǎn)品使用。 |
19156044901 （同微信）
the mRNA levels of TGF- β1 and TIMP－2，and the protein levels of TGF- β1，TIMP－2，
and p- β-catenin / β-catenin were obviously increased (P＜0．05) ，the corresponding index of TAK－715 group showed the opposite trends ( P＜0．05) ;
compared with the ATR group，the axial length，refraction，the levels of IL－1β , IL－6 and TNF- α , the mRNA level of MMP－2，
and the protein lev- els of Wnt and MMP－2 in the ATR+TAK－715 group were obviously
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