IPEC-1豬小腸上皮細(xì)胞、I
- 公司名稱 上海富雨生物科技有限公司
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產(chǎn)品名稱：IPEC-1豬小腸上皮細(xì)胞、IPEC-1豬小腸上皮細(xì)胞、IPEC-1豬小腸上皮細(xì)胞、IPEC-1豬小腸上皮細(xì)胞；
IPEC-1 豬小腸上皮細(xì)胞
Catalogue No.: C1018
Product Format: a T25 flask
Culture Properties：貼壁
Complete Growth Medium: 89%1640+10%FBS+1%雙抗
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Application: Cells and cancer research
NOTE: FOR RESEARCH USE ONLY.
Components
| Item | Specifications |
| a T25 flask | 2X106 |
| Manual | 1 copy |
For the intracellular molecules, cells were ?xed and permeabilized using ?xation and permeabilization buffer (BD Biosciences) for 15 min at room temperature ?rst. After two washes with perm/wash buffer (BD Biosciences), the cells were stained with intracellular antibodies in wash buffer including Ki67 (Clone: B56) and IFN-Y (Clone: B27) (Source: BD Biosciences, USA) for 15 min without light exposure. After two washes with PBS, the cells were loaded on a ?ow cytometry machine for detection. Speci?cally, intracellular IFN-Y detection was required prior to overnight operation by GolgiPlug in 1: 1000 work concentration, a protein transporter inhibitor (BD Biosciences, USA).
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