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化工儀器網(wǎng)>產(chǎn)品展廳>試劑標物>常用實驗試劑>基準試劑>500ML- BCA Protein Assay Reagent,BCA蛋白定量試劑...

500ML- BCA Protein Assay Reagent,BCA蛋白定量試劑盒

參考價 1843
訂貨量 ≥1
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  • 公司名稱 上海葉舟生物科技有限公司
  • 品牌
  • 型號 500ML-
  • 產(chǎn)地 Pierce 23225
  • 廠商性質(zhì) 經(jīng)銷商
  • 更新時間 2018/2/24 15:52:09
  • 訪問次數(shù) 2695

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G418,MTT,IPTG,DTT,STZ,ONPG

BCA蛋白定量試劑盒

中文名:BCA (tm) Protein Assay Kit

英文名BCA蛋白定量試劑盒

Pierce相關(guān)資料如下:

BCA Protein Assay Reagent (bicinchoninic acid) 

Used in more labs than any other detergent-compatible protein assay.

The Thermo Scientific Pierce BCA Protein Assay remains one of the most popular protein assay methods worldwide. The detergent-compatible method provides for accurate determination of protein concentration with most sample types encountered in protein research laboratories. The Pierce BCA Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

Highlights:

  • Colorimetric method; read at 562 nm
  • Compatible with most ionic and nonionic detergents
  • Faster and easier than the Lowry method
  • Working reagent stable for 24 hours
  • Linear working range for BSA from 20-2,000 µg/ml
  • Minimum detection level of 5 µg/ml with the enhanced protocol
  • Adaptable to microplates
  • Less protein-to-protein variation than dye-binding methods

BCA Protein Assay Applications:

  • Studying protein:protein interactions
  • Measuring column fractions after affinity chromatography
  • Estimating percent recovery of membrane proteins from cell extracts
  • High-throughput screening of fusion proteins

Substances compatible with the Thermo Scientific BCA Protein Assay. The following is a short list of compatible substances and their concentrations that the BCA Protein Assay can tolerate. For a more complete list please refer to the instruction booklet for the product or view our Table of Compatible Substances for a comparison of substance compatibilities among several different protein assay methods.

Substance

Compatible Conc.

Substance

Compatible Conc.

NP-40

5.0%

Glucose (fresh)

10 mM

Emulgen

1.0%

EDTA

10 mM

Hepes

100 mM

Sodium Chloride

1.0 M

DTT

1 mM

NaOH

0.1 M

Guanidine•HCl

4.0 M

Ammonium Sulfate

1.5 M

Triton X-100

5.0%

Sodium Acetate, pH 5.5

200 mM

Octyl-ß-Glucoside

5.0%

SDS

5.0%

Urea

3.0 M

Brij-35

5.0%

Sucrose (fresh)

40%

Lubrol

1.0%

Glycine, pH 2.8

100 mM

CHAPS

5.0%

Please refer to the product instructions for special notes and definitions about compatibility tolerances.

 

Time comparison of BCA Protein Assay Reagent vs. Lowry Protein Assay.

BCA Protein Assay Reagent
1. Mix reagents 1 minute
2. Add sample and incubate 30 minutes
3. Read at 562 nm 1 minute
Total BCA Time: 32 minutes

Lowry Protein Assay
1. Make reagents 70 minutes
2. Add sample and incubate 20 minutes
3. Add Folin Reagent 1 minute
4. Incubate 30 minutes
5. Read at 750 nm 1 minute
Total Lowry Time: 122 minutes

 

 

Standard curves. Typical standard curves for bovine serum albumin (BSA) and bovine gamma globulin (BGG) in the BCA Protein Assay. Kits include ampules of Albumin Standard.

How the BCA Protein Assay Detects Protein:

The BCA Protein Assay combines the well-known reduction of Cu2+ to Cu1+ by protein in an alkaline medium with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu1+) by bicinchoninic acid. The first step is the chelation of copper with protein in an alkaline environment to form a light blue complex. In this reaction, known as the biuret reaction, peptides containing three or more amino acid residues form a colored chelate complex with cupric ions in an alkaline environment containing sodium potassium tartrate.

In the second step of the color development reaction, bicinchoninic acid (BCA) reacts with the reduced (cuprous) cation that was formed in step one. The intense purple-colored reaction product results from the chelation of two molecules of BCA with one cuprous ion. The BCA/copper complex is water-soluble and exhibits a strong linear absorbance at 562 nm with increasing protein concentrations. The BCA reagent is approximay 100 times more sensitive (lower limit of detection) than the pale blue color of the first reaction.

The reaction that leads to BCA color formation is strongly influenced by four amino acid residues (cysteine or cystine, tyrosine, and tryptophan) in the amino acid sequence of the protein. However, unlike the Coomassie dye-binding methods, the universal peptide backbone also contributes to color formation, helping to minimize variability caused by protein compositional differences.

For more information, see the article "Chemistry of Protein Assays" in the Protein Methods Library.

References:

1.       Smith, P.K., et al. (1985). Anal. Biochem. 150, 76-85.

2.       Sorensen, K. (1992). BioTechniques 12(2), 235-236.

3.       Ju, T., et. al. (2002). J. Biol. Chem. 277, 178-186.

4.       Shibuya, T., et. al. (1989). Tokyo Ika Daigaku Zasshi 47(4), 677-682.

5.       Hinson, D.L. and Webber, R.J. (1988). BioTechniques 6(1), 14, 16, 19.

6.       Akins, R.E. and Tuan, R.S. (1992). BioTechniques 12(4), 496-7, 499.

7.       Tylianakis, P.E., et. al.(1994). Anal. Biochem. 219(2), 335-340.

8.       Gates, R.E. (1991). Anal. Biochem. 196(2), 290-295.

9.       Stich, T.M. (1990). Anal. Biochem. 191, 343-346.

10.   Tuszynski, G.P. and Murphy, A. (1990). Anal. Biochem. 184(1), 189-191.

Related Links:
BCA Protein Assay Reagents FAQ
Protein:Protein Variation of Protein Assays
Protein Assay Selection Guide

Protein Methods Library:
Overview of Protein Assays
Chemistry of Protein Assays
Protein Assay Data Analysis

Compatible Instrumentation:
BioMate 3 UV-Vis Spectrophotometer
BioMate 5 UV-Vis Spectrophotometer
NanoDrop 2000c UV/Vis Spectrophotometer
NanoDrop 8000 UV/Vis 8 Sample Spectrophotometer

Related Products:
BSA & BGG Protein Assay Standard Sets
Micro BCA Protein Assay Kit
BCA Protein Assay - Reducing Agent Compatible
Coomassie Plus (Bradford) Protein Assay
Pierce 660 nm Protein Assay

 

Ordering Information

Certificate of Analysis    Product Instructions    MSDS

Buy

Product #

Description

Pkg. Size

Files

Price

Local contact

23225

BCA Protein Assay Kit    現(xiàn)貨
Sufficient reagents to perform 500 standard tube assays or 5,000 microplate assays.
Kit Contents:
Reagent A, 2 x 500 ml
Reagent B, 25 ml
Albumin Standard Ampules (2 mg/ml), 10 x 1 ml

Kit

Local contact

Local contact

23227

BCA Protein Assay Kit
Sufficient reagents to perform 250 standard tube assays or 2,500 microplate assays.
Kit contains:
Reagent A, 1 x 500 ml
Reagent B, 25 ml
Albumin Standard Ampules (2 mg/ml), 10 x 1 ml

Kit

Local contact

Local contact

23221

BCA Protein Assay Reagent A
Bicinchoninic acid and tartrate in an alkaline carbonate buffer.
Sufficient for 1,250 microplate assays.

250 ml

Local contact

Local contact

23228

BCA Protein Assay Reagent A
Bicinchoninic acid and tartrate in an alkaline carbonate buffer.
Sufficient for 2,500 microplate assays.

500 ml

Local contact

Local contact

23223

BCA Protein Assay Reagent A
Bicinchoninic acid and tartrate in an alkaline carbonate buffer.
Sufficient for 5,000 microplate assays.

1 L

Local contact

Local contact

23222

BCA Protein Assay Reagent A
Bicinchoninic acid and tartrate in an alkaline carbonate buffer.
Sufficient for 18,750 microplate assays.

3.75 L

Local contact

Local contact

23224

BCA Protein Assay Reagent B
4% solution of copper sulfate pentahydrate.
Sufficient for 5,000 microplate assays.

25 ml

Local contact

Local contact

23230

BCA Solid
Bicinchoninic acid as a recrystallized purified powder.
Sufficient to make 2.5L of Reagent A.

25 g

Local contact

 

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