化工儀器網(wǎng)>產(chǎn)品展廳>試劑標(biāo)物>行業(yè)專用試劑>生化與分子生物學(xué)用試劑>Roche03353583910原裝 羅氏地高辛標(biāo)記檢測(cè)試劑盒
Roche03353583910原裝 羅氏地高辛標(biāo)記檢測(cè)試劑盒
參考價(jià) | ¥ 5034 |
訂貨量 | ≥1 |
- 公司名稱 廣州市齊云生物技術(shù)有限公司
- 品牌
- 型號(hào) Roche03353583910原裝
- 產(chǎn)地 美國(guó)羅氏生物科學(xué)供應(yīng)部
- 廠商性質(zhì) 代理商
- 更新時(shí)間 2018/2/19 10:20:25
- 訪問(wèn)次數(shù) 744
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生化試劑,標(biāo)準(zhǔn)品,ELISA試劑盒,RT-PCR相關(guān)試劑,基因檢測(cè)試劑,農(nóng)業(yè)殘留檢測(cè)試劑等
Catalog # | Pack size | Qty | Price | Options |
---|---|---|---|---|
1 kit for 25 tailing reactions 100 pmol oligonucleotide per assay corresponding to 1 μg of a 30-mer oligonucleotide | 5034.00 |
一、使用原理:
Oligonucleotides, tailed with DIG-dUTP, are used for DNA or RNA hybridization in membrane blots or in situ hybridization.
二、產(chǎn)品優(yōu)點(diǎn):
- Very sensitive hybridization probes, due to the incorporation of several DIG-nucleotides
- Fast hybridization kinetics, due to the small size of oligonucleotides
- Single-stranded probes, no renaturation during hybridization
- Sequence can be designed according to the experiment
- Specially suited for in situ hybridization; due to their small size, oligonucleotides readily diffuse into fixed tissues and cells
三、產(chǎn)品描述:
Tailing of oligonucleotides at the 3'-end with DIG-11-dUTP and recombinant Terminal Transferase. Oligonucleotides are tailed with DIG-dUTP and dATP at an average tail length of 50 nucleotides (tail length range: 10 – 100).
四、試劑盒組成組分:
- Reaction Buffer, 5x, 100 µl
- CoCl2 Solution, 100 µl, 25 mM
- DIG-dUTP Solution, 25 µl, 1 mM
- dATP Solution, 25 µl, 10 mM
- Recombinant Terminal Transferase, 25 µl, [400 U/µl]
- Control Oligonucleotide, unlabeled, 25 µl, [20 pmol/µl]
- Oligonucleotide, DIG-dUTP/dATP tailed, 20 µl, [2.5 pmol/µl]
- Control DNA, 20 µl, [0.25 mg/ml]
- Glycogen Solution, 50 µl, [20 mg/ml]
- DNA Dilution Buffer, 3 x 1 ml, [50 µg/ml] fish sperm DNA
- poly(A) Solution, 1 ml, [10 mg/ml]
五:試劑盒檢測(cè)原理:
相關(guān)分類
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