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CYT-244

重組人肝細胞生長因子（rHuHGF）

2μg/10μg/1mg

Prospec

Background:
	Hepatocyte Growth Factor （HGF） is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3 and GM-CSF to stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis
	Description :
	Recombinant Human HGF produced in Baculovirus is a heterodimer, non-glycosylated, polypeptide chain consisting an -chain of 463 amino acids and -chain of 234 having a total molecular mass of 80017 Dalton. HGF is purified by proprietary chromatographic techniques.
	Physical Appearance:
	Sterile Filtered White lyophilized （freeze-dried） powder.
	Formulation:
	The sterile protein powder is lyophilized with no additives.
	Solubility:
	It is recommended to reconstitute the lyophilized HGF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
	Stability:
	Lyophilized HGF although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution HGF should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles.
	Purity:
	Greater than 98.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
	Amino acid sequence:
	Agrees with the sequence of native human HGF.
	Dimers and aggregates:
	Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
	Biological Activity:
	ProSpec's Hepatocyte Growth Factor is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of monkey 4MBr-5 indicator cells was found to be 20-40 ng/ml.
	Endotoxin:
	Less than 0.1 ng/µg （IEU/µg） of HGF.
	Protein content:
	Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.83 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of HGF as a Reference Standard.
	Usage:
	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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