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OVCAR-3 人卵巢腺癌細(xì)胞的介紹

時(shí)間：2014-8-22閱讀：4643

OVCAR-3 人卵巢腺癌細(xì)胞的介紹

形態(tài)特性：上皮細(xì)胞樣
生長(zhǎng)特性：貼壁生長(zhǎng)
特征特性：OVCAR-3細(xì)胞源自一名60歲白人女性卵巢腫瘤組織，由T.C.Hamiltonyu建系于1982年。該細(xì)胞帶有雌、雄激素受體。對(duì)阿霉素，順氯氨鉑，(左旋)苯丙氨酸氮芥有一定抗藥性，適用于卵巢癌的抗藥性研究。OVCAR-3染色體數(shù)量在亞三倍體范圍內(nèi)。
傳代方法： 1:3傳代,2-3天傳一代
支原體檢測(cè)：陰性
此細(xì)胞為我公司走ATCC保藏中心引進(jìn)，以下是ATCC介紹
NIH:OVCAR-3 [OVCAR3] (ATCC^® HTB-161^™)

Organism	Homo sapiens, human
Tissue	ovary
Cell Type	epithelial
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	1
Disease	adenocarcinoma
Age	60 years
Gender	female
Ethnicity	Caucasian
Applications	This cell line is a suitable transfection host. NIH:OVCAR-3 is an appropriate model system in which to study drug resistance in ovarian cancer, and the presence of hormone receptors should be useful for the evaluation of hormonal therapy.
Storage Conditions	liquid nitrogen vapor temperature

Derivation	The NIH:OVCAR-3 line was established in 1982 by T.C. Hamilton, et al. from the malignant ascites of a patient with progressive adenocarcinoma of the ovary.
Clinical Data	Caucasian female 60 years
Receptor Expression	Androgen receptor, positive; estrogen receptor, positive; progesterone receptor, positive
Tumorigenic	Yes
Effects	Yes, Forms colonies in soft agar Yes, in nude mice inoculated subcutaneously with 10(7) cells (Tumors developed within 21 days at 100% frequency (5/5).)
Comments	Forms colonies in soft agar and has an abnormal karyotype. Resistant to clinically relevant concentrations of adriamycin, melphalan and cisplatin. Both cultured cells and xenografts exhibit androgen and estrogen receptors. Xenograft models have been used to show that treatment with 17 beta estradiol can induce progesterone receptors in this human ovarian carcinoma.

Karyotype	The cell line is aneuploid human female, with chromosome counts in the sub to near-triploid range. Several normal chromosomes (N11, N13, N14, N15, N16, N17, and N22) are clearly under-represented. Many of these missing chromosomes are represented in the large number of cytogenetically altered chromosomes identified as marker chromosomes. In addition to the marker chromosomes, there are a large number of other structurally abnormal and unassignable chromosomes that are not recognized as markers. Random loss and gain of chromosomes from cell to cell are noted in the exact chromosome counts and in the analysis of the karyotypes.

Karyotype

The cell line is aneuploid human female, with chromosome counts in the sub to near-triploid range. Several normal chromosomes (N11, N13, N14, N15, N16, N17, and N22) are clearly under-represented. Many of these missing chromosomes are represented in the large number of cytogenetically altered chromosomes identified as marker chromosomes. In addition to the marker chromosomes, there are a large number of other structurally abnormal and unassignable chromosomes that are not recognized as markers. Random loss and gain of chromosomes from cell to cell are noted in the exact chromosome counts and in the analysis of the karyotypes.