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上海恒遠(yuǎn)生物科技有限公司>技術(shù)文章>CAMP抗體說(shuō)明書(shū)

技術(shù)文章

CAMP抗體說(shuō)明書(shū)

閱讀:1847          發(fā)布時(shí)間:2012-5-29

 

 
 Goat Anti-CAMP
Catalog Number :HZ99740Quantity size : 0.1ml (dilute with pH 7.4
0.01 M PBS or antibody diluent ) Background: Phosphodiesterases (PDE, also designated cyclic nucleotide phosphodiesterase) are important for the downregulation of the intracellular level of the second messenger cyclic adenosine monophosphate (cAMP) by hydrolyzing cAMP to 5'AMP. The PDE family contains proteins that serve tissue-specific roles in the regulation of lipolysis, glycogenolysis, myocardial contractility and smooth muscle relaxation. PDE, also designated cGMP-binding cGMP-specific phosphodiesterase or CGB-PDE, regulates the intracellular concentration of cyclic nucleotides and thereby is important in signal transduction. PDE catalyzes the hydrolysis of cGMP to 5’GMP and the protein is expressed in heart, placenta, aortic smooth muscle cells, skeletal muscle and pancreas.
 
 
pecificity :
              Anti-CAMPis a goat polyclonal antibody unconjugated .
              specific for CAMPof human, mouse, rat and rabbit
              use for western blotting,elisa,immunoprecipitation and immunohistochemistry
              Protein G affinity chromatography purification, purity :>95%
              Isotype: IgG
 
Application :
              Western blotting 1:100-500
              Immunohistochemistry 1:100-500
              ELISA 1:500-1000
              Optimal working dilutions must be determined by the end user. Storage: Store at -20for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4
 
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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