化工儀器網(wǎng)
資料下載
恒遠(yuǎn)產(chǎn)品文獻(xiàn):雞GP,GS,GK等指標(biāo)ELISA試劑盒引用文獻(xiàn)
閱讀：1007 發(fā)布時(shí)間：2023-8-21| 提 供 商 | 上海恒遠(yuǎn)生物科技有限公司 | 資料大小 | 1.7MB |
|---|---|---|---|
| 資料圖片 | 下載次數(shù) | 7次 | |
| 資料類(lèi)型 | PDF 文件 | 瀏覽次數(shù) | 1007次 |
| 免費(fèi)下載 | 點(diǎn)擊下載 |
【文獻(xiàn)標(biāo)題】Hydroxycitric Acid Reduced Lipid Droplets Accumulation Via Decreasing Acetyl-Coa Supply and Accelerating Energy Metabolism in Cultured Primary Chicken Hepatocytes
【作者】Longlong Li ,Mengling Peng ,Chongyang Ge, et.al
【作者單位】南京農(nóng)業(yè)大學(xué)（Nanjing Agricultural University）
【文獻(xiàn)中引用產(chǎn)品】
雞糖原磷酸化酶(GP)ELISA試劑盒
雞糖原合成酶(GS)ELISA試劑盒
雞葡萄糖激酶(GK)ELISA試劑盒
雞磷酸果糖激酶-1（PFK-1）ELISA試劑盒
雞丙酮酸激酶（PK）ELISA試劑盒
雞丙酮酸脫氫酶E1(PDH E1)ELISA試劑盒
雞檸檬酸合成酶(CS)ELISA試劑盒
雞烏頭酸酶(ACO)ELISA試劑盒
雞磷酸烯醇式丙酮酸羧激酶（PEPCK）ELISA試劑盒
雞ATP-檸檬酸裂解酶(ACLY)ELISA試劑盒
雞NADH脫氫酶ELISA試劑盒
雞ATP合酶（ATP）ELISA試劑盒
【關(guān)鍵詞】Hydroxycitric acid Acetyl-CoA ATP-citrate ? Energy metabolism Primary chicken hepatocytes
【DOI】 10.1159/000481564
【影響因子(IF)】11.7
【出版期刊】《CELLULAR PHYSIOLOGY AND BIOCHEMISTRY》
【產(chǎn)品原文引用】
Measurement of the key glucose metabolic enzyme protein content
Cells were cultured in 6-well plates (2×106 cells per well) and treated with 0, 1, 10 or 50 μM (-)-HCA for 24 h. The cells were harvested and disrupted ultrasonically in ice and then centrifuged at 3000 rpm for 20 min at 4 °C. The supernatants were collected, the protein concentrations of glycogen phosphorylase (GP) (catalog #: E-75175), glycogen synthase (GS) (catalog #: E-75174), glucokinase (GK) (catalog #: E-76111), phosphofructokinase-1 (PFK-1) (catalog #: E-76131), pyruvate kinase (PK) (catalog #: E-76565), pyruvate dehydrogenase (PDH, E1) (catalog #: E-76118), citrate synthase (CS) (catalog #: E-75165), aconitase (ACO) (catalog #: E-75144) and phosphoenolpyruvate carboxykinase (PEPCK) (catalog #: E-75117) were measured using ELISA kits according to the manufactures’ protocols (Shanghai Hengyuan Biological Technology Co., China). The enzyme protein content was normalized to total protein concentration in the sample.
完整版PDF文獻(xiàn)請(qǐng)咨詢?cè)诰€客服或者聯(lián)系我司業(yè)務(wù)員！
更多公司福利請(qǐng)關(guān)注“恒遠(yuǎn)生物"微信公眾號(hào)！