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【文獻(xiàn)標(biāo)題】Dietary sodium lactate promotes protein and lipid deposition through increasing energy supply from glycolysis in Nile tilapia (Oreochromis niloticus) 

【作者】Rui-Xin Li , Wen-Hao Zhou , Jiong Ren , et.al

【作者單位】華東師范大學(xué)（East China Normal University）

【文獻(xiàn)中引用產(chǎn)品】

魚胰島素(Insulin)ELISA試劑盒

【關(guān)鍵詞】Lactate ,Growth performance ,Metabolism ,Energy homeostasis, Nile tilapia 

【DOI】doi.org/10.1016/j.aquaculture.2021.737858

【影響因子(IF)】5.19

【出版期刊】《Aquaculture》

【產(chǎn)品原文引用】

For the biochemical parameter measurement, serum or/and hepatic pyruvate, lactate, free fatty acids, triglyceride, glucose and glycogen were measured using commercial kits (Jiancheng Biotech Co. China) according to the instructions. Briefly, 2–4-dinitrophenylhydrazine was specifically reacted with pyruvate to produce a red color at acid condition. Lactate was oxidized by lactate dehydrogenase to generate a product which interacted with a tetrazolium salt WST-8 dye to form a colorimetric (450 nm) product. Free fatty acids (FFA) were measured by a coupled enzymatic reaction system (ACS-ACOD Method). First, acyl CoA synthetase (ACS) catalyzed fatty acid acylation of coenzyme A. Next, the acyl-CoA product was oxidized by acyl CoA oxidase (ACOD), producing hydrogen peroxide which reacted with color agent. Triglycerides were detected with GPO/PAP (glycerol phosphate oxidase/ peroxidase and hydrogen peroxide) method. Glucose was oxidized by glucose oxidase into D-gluconic acid and hydrogen peroxide, which was detected with a highly specific colorimetric probe. Under the presence of concentrated sulfuric acid, glycogen was dehydrated to furfural derivatives and the furfural derivatives could form blue compound with anthracenone. The serum insulin was assessed by ELISA (Enzyme Linked Immunosorbent Assay) kit (Hengyuan Biotech Co. China) according to the manufacturer’s protocols. 

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