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滬鼎產(chǎn)品文獻:植物吲哚乙酸(IAA)ELISA試劑盒引用文獻

【文獻標題】Overexpression of the LcCUC2-likegene in Arabidopsis thaliana alters thecotyledon morphology and increases rosette leaf number

【作者】Shaoying Wen1,*, Jiayu Li1,*, Ziyuan Hao1, 

【作者單位】南京林業(yè)大學

【DOI】 DOI 10.7717/peerj.12615

【影響因子(IF)】2.98

【文獻中引用產(chǎn)品】

植物吲哚乙酸(IAA)ELISA試劑盒

【出版期刊】《Overexpression of the LcCUC2-likegene in Arabidopsis thaliana alters thecotyledon morphology and increases  rosette leaf number

【產(chǎn)品原文引用】Auxin content measurement

Basal rosette leaves samples of 0.6 g were harvested from 3-week-old WT and LcCUC2L

transgenic A. thaliana plants and immediately frozen in liquid nitrogen. Determination

of indole-3-acetic acid (IAA) in A. thaliana leaves was performed by ELISA (Huding,

Shanghai, China). Three replicates were used for each sample.

Histochemical staining to detect GUS activity

The β-Galactosidase Reporter Gene Staining Kit (Leagene) was used for GUS histochemical

staining in ProLcCUC2L transgenic and WT A. thaliana plants. In this study, seven-day-old

seedlings and fourteen-day-old seedlings were processed. Moreover, leaves at different

stages of development (10, 20 and 30 days of age) from plants planted in soil were

subjected to GUS staining. All materials were treated with GUS staining solution and

incubated overnight at 37 ?C, followed by bleaching with 75% (v/v) ethanol.

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【產(chǎn)品文獻支持】Auxin content measurement

Basal rosette leaves samples of 0.6 g were harvested from 3-week-old WT and LcCUC2L

transgenic A. thaliana plants and immediately frozen in liquid nitrogen. Determination

of indole-3-acetic acid (IAA) in A. thaliana leaves was performed by ELISA (Huding,

Shanghai, China). Three replicates were used for each sample.

影響因子(IF)= ：2.98 標題:大學:南京林業(yè)大學《Nanjing Forestry University》