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[供應(yīng)]Cellartis Y30050 Hepatocyte Differentiation Kit 分化獲取肝細(xì)胞試劑盒
華雅旗下紅榮微再*代理（上海）TAKARA CellArtis，Rubicon產(chǎn)品 詳詢 1500 1904 520 紅榮微再-客服: 經(jīng)銷商專員
Cellartis-Y30050 Cellartis Hepatocyte Differentiation Kit
產(chǎn)品英文名稱：Cellartis Hepatocyte Differentiation Kit
產(chǎn)品中文名稱：Cellartis分化獲取肝細(xì)胞試劑盒
用于誘導(dǎo)內(nèi)胚層細(xì)胞（definitive endoderm (DE)）獲取肝細(xì)胞
產(chǎn)品概述：
The Cellartis Hepatocyte Differentiation Kit用于誘導(dǎo)內(nèi)胚層細(xì)胞（definitive endoderm (DE)）獲取肝細(xì)胞。通過使用本產(chǎn)品獲取的肝細(xì)胞表現(xiàn)出符合預(yù)期的代謝酶表達(dá)，從而可以用于藥物代謝研究和毒理學(xué)篩選。本產(chǎn)品包含足夠的維持培養(yǎng)基用于正常為期11天的實(shí)驗(yàn)。
The Cellartis Hepatocyte Differentiation Kit可以對(duì)廣泛來源的DE細(xì)胞進(jìn)行定向分化：客戶自己的DE細(xì)胞，或是Cellartis提供的標(biāo)準(zhǔn)化DE細(xì)胞，或是客戶利用Cellartis Definitive Endoderm Differentiation Kit獲得的DE細(xì)胞。據(jù)估計(jì)，現(xiàn)在已經(jīng)有28種發(fā)源于人類干細(xì)胞的DE細(xì)胞被成功測試可以使用本產(chǎn)品。特別地，如果客戶是用于大規(guī)模、要求持續(xù)穩(wěn)定獲得肝細(xì)胞的相關(guān)用途，Cellartis推薦Definitive Endoderm Cells (from ChiPSC18)，用作起始DE細(xì)胞材料，或是用作陽性對(duì)照品。如果客戶要求的DE是來自于自有的樣本材料（patient-specific, or disease-specific），建議使用Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055)。
The Cellartis Hepatocyte Differentiation Kit generates highly homogeneous hepatocyte cultures starting from definitive endoderm (DE) cells. The resulting hepatocytes show the expected expression of relevant metabolic enzymes, making them suitable for drug metabolism studies and toxicity screening. The Cellartis Hepatocyte Differentiation Kit contains sufficient maintenance medium to perform experiments in an 11-day time window.
There are several options for source materials to generate functional hepatocytes: your own DE cells, our DE cells, or DE cells you generate using the Cellartis Definitive Endoderm Differentiation Kit. This kit has been successfully tested with DE cells derived from 28 different human pluripotent cell lines. Our cells, Cellartis Definitive Endoderm Cells (from ChiPSC18), are suitable as the starting material or as a positive control. If you desire a customized (standard, patient-specific, or disease-specific) genetic background for your DE cells, use the complete Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055).
產(chǎn)品特點(diǎn):
完整的試劑盒 - 包括四種不同的培養(yǎng)基和即用包被，足以進(jìn)行為期23天的方案，將定形內(nèi)胚層（DE）細(xì)胞分化為肝細(xì)胞
方法可靠 - 使用超過25種不同的干細(xì)胞系測試過
有效的分化 - 產(chǎn)生> 90％純度的肝細(xì)胞
Complete kit—includes four different media sets and a ready-to-use coating, sufficient for a 23-day protocol for differentiating definitive endoderm (DE) cells into hepatocytes
Robust protocol—tested with more than 25 different stem cell lines
Efficient differentiation—yields >90% pure hepatocytes
組份： –20°C保存
包括解凍接種培養(yǎng)基、祖代培養(yǎng)基、成熟培養(yǎng)基及添加劑、維持培養(yǎng)基和包被劑
1 bottle Hepatocyte Thawing and Seeding Medium (1) (25 ml)
1 bottle Hepatocyte Progenitor Medium (2) (50 ml)
2 bottles Hepatocyte Maturation Medium Base (3A) (24 ml)
1 tube Hepatocyte Maturation Medium Supplement (3B) (2.6 ml)
3 bottles Hepatocyte Maintenance Medium (4) (50 ml)
1 tube Hepatocyte Coating (7.5 ml)
自備試劑：
人類定形內(nèi)胚層細(xì)胞（推薦Cellartis 的DE細(xì)胞）
胎牛血清（FBS）或敲除血清替代品（KO-SR）
PBS Dulbecco's不含Ca 2+和Mg 2+（D-PBS - / - ）
TrypLE選擇酶（1X），不含酚紅
產(chǎn)品性能：
Definitive endoderm (DE) cells were differentiated into hepatocytes using the Cellartis Hepatocyte Differentiation Kit. The first field of view, labeled “Definitive Endoderm Day 7,” shows DE cells seven days after differentiation from human pluripotent cells. These DE cells became the starting material for the differentiation kit. The remaining progression from DE cells to hepatocytes shows the expected morphology over a 14-day time period. The morphological features present in the last field of view, labeled “Hepatocyte Day 21+,” continue stably past Day 21.
人iPS誘導(dǎo)分化成肝細(xì)胞全過程的完整試劑盒：Y30055（新產(chǎn)品）
人iPS來源肝細(xì)胞株及*培養(yǎng)基Y10133/Y10134/Y10135（新產(chǎn)品）
人原代肝臟細(xì)胞培養(yǎng)基 Y20020（新產(chǎn)品）
http://www.duoo135.com/st321032/article_1496885.html
華雅再生醫(yī)學(xué)旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司主營細(xì)胞治療和精準(zhǔn)醫(yī)療等產(chǎn)品，2018年與TAKARA繼續(xù)合作，*代理（上海）其旗下Cellartis干細(xì)胞研究制品和Rubicon單細(xì)胞文庫構(gòu)建試劑。Cellartis公司位于瑞典，擁有iPS細(xì)胞等干細(xì)胞分化為肝細(xì)胞及臟器細(xì)胞的分化誘導(dǎo)技術(shù)和ES細(xì)胞，ips細(xì)胞，分化細(xì)胞等干細(xì)胞相關(guān)產(chǎn)品。
Cellartis-Y30050 Cellartis Hepatocyte Differentiation Kit 手機(jī)1500 1904 520 紅榮微再-客服 經(jīng)銷商專員
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