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CD4(T細(xì)胞)兔單克隆抗體

廣州健侖生物科技有限公司

CD4主要分布在輔助T細(xì)胞/誘導(dǎo)T細(xì)胞以及吞噬細(xì)胞，正常淋巴組織中，CD4的表達(dá)數(shù)量明顯多于CD8，其比例約為4：1，主要用于皮膚T細(xì)胞淋巴瘤和菌樣霉菌病以及對T細(xì)胞亞群的研究，對后者不能作為區(qū)別腫瘤性或反應(yīng)性T細(xì)胞的參考依據(jù)。

我司還提供其它進(jìn)口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細(xì)菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【產(chǎn)品介紹】

細(xì)胞定位：細(xì)胞膜/細(xì)胞漿

克隆號：SP35

適用組織：石蠟/冰凍

陽性對照：扁桃體

抗原修復(fù)：熱修復(fù)（EDTA）

抗體孵育時間：30-60min

CD4(T細(xì)胞)兔單克隆抗體

注意：因Lowry反應(yīng)的顯色隨時間不斷加深，因此各項操作必須精確控制時間，即第1支試管加入5毫升試劑甲后，開始計時，1分鐘后，第2支試管加入5毫升試劑甲，2分鐘后加第3支試管，余此類推。全部試管加完試劑甲后若已超過10分鐘，則第1支試管可立即加入0.5毫升試劑乙，1分鐘后第2支試管加入0.5毫升試劑乙，2分鐘后加第3支試管，余此類推。待zui后一支試管加完試劑后，再放置30分鐘，然后開始測定光吸收。每分鐘測一個樣品。
進(jìn)行多試管操作時，為了防止出錯，每位學(xué)生都必須在實驗記錄本上預(yù)先畫好下面的表格。表中是每個試管要加入的量（毫升），并按由左至右，由上至下的順序，逐管加入。zui下面兩排是計算出的每管中蛋白質(zhì)的量（微克）和測得的吸光度值。
2.樣品的測定：取1毫升樣品溶液（其中約含蛋白質(zhì)20~250微克），按上述方法進(jìn)行操作，取1毫升蒸餾水代替樣品作為空白對照。通常樣品的測定也可與標(biāo)準(zhǔn)曲線的測定放在一起，同時進(jìn)行。即在標(biāo)準(zhǔn)曲線測定的各試管后面，再增加3個試管。如上表中的8、9、10試管。
根據(jù)所測樣品的吸光度值，在標(biāo)準(zhǔn)曲線上查出相應(yīng)的蛋白質(zhì)量，從而計算出樣品溶液的蛋白質(zhì)濃度。
注意，由于各種蛋白質(zhì)含有不同量的酪氨酸和苯丙氨酸，顯色的深淺往往隨不同的蛋白質(zhì)而變化。因而本測定法通常只適用于測定蛋白質(zhì)的相對濃度（相對于標(biāo)準(zhǔn)蛋白質(zhì)）。
[實驗?zāi)康腯
1. 學(xué)習(xí)血紅蛋白電泳分離方法；
2. 能辨認(rèn)正常人和異常血紅蛋白的電泳圖譜；

CD4(T細(xì)胞)兔單克隆抗體

我司還提供其它進(jìn)口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細(xì)菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】     歐

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【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

Note: Due to the Lowry reaction color development over time, so the operation must be precisely control the time, that is, after the first tube added 5 ml of reagent A, the start time, 1 minute later, the second tube 5 ml of reagent A , 2 minutes later add the third test tube, and so on. If the test tube has been filled for more than 10 minutes, add the first test tube to 0.5 ml of reagent B, add the second test tube to 0.5 ml of reagent B after 1 minute, and add the third test tube after 2 minutes. analogy. After the last test tube plus reagents, and then placed for 30 minutes, and then began to measure the light absorption. A sample is measured every minute.
For multiple test tube operations, each student must pre-draw the following table in the lab log in order to prevent errors. The table is the amount of each test tube to be added (ml), and by pipe from left to right, from top to bottom, by tube. The bottom two rows are the calculated amounts of protein (in micrograms) per tube and the measured absorbance values.
2. Sample Determination: Take 1 ml of sample solution (which contains about 20 to 250 micrograms of protein), according to the above method to operate, take 1 ml of distilled water instead of the sample as a blank control. Usually the determination of the sample can also be measured with the standard curve put together at the same time. That is, after each test tube determined by the standard curve, another 3 test tubes are added. As in the above table 8,9,10 test tube.
According to the absorbance value of the sample measured, the corresponding protein amount was found on the standard curve to calculate the protein concentration of the sample solution.
Note that because various proteins contain different amounts of tyrosine and phenylalanine, the color depth tends to vary with different proteins. Therefore, this assay is usually only suitable for the determination of the relative concentration of the protein (relative to the standard protein).
[Purpose]
1. Learn hemoglobin electrophoresis separation method;
2. Can identify normal and abnormal hemoglobin electrophoresis patterns;