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CD30(Ki-1抗原)鼠單克隆抗體

廣州健侖生物科技有限公司

CD30 是一種分子量為120KDa的跨膜單鏈糖蛋白，是細(xì)胞因子配體CD30L的受體，在淋巴細(xì)胞活化中起重要作用。CD30表達(dá)于活化的 T/B 細(xì)胞、R-S細(xì)胞、部分淋巴濾泡周?chē)霓D(zhuǎn)化細(xì)胞、大多數(shù)間變性大細(xì)胞淋巴瘤細(xì)胞，在胚胎性癌中也發(fā)現(xiàn)CD30表達(dá)。此抗體主要用于研究R-S細(xì)胞和大多數(shù)間變性大細(xì)胞淋巴瘤。

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【產(chǎn)品介紹】

細(xì)胞定位：細(xì)胞膜

克隆號(hào)：Ber-H2

同型：IgG1/K

適用組織：石蠟/冰凍

陽(yáng)性對(duì)照：霍奇金氏淋巴瘤

抗原修復(fù)：熱修復(fù)（EDTA）

抗體孵育時(shí)間：30-60min

CD30(Ki-1抗原)鼠單克隆抗體

（一）實(shí)驗(yàn)原理
雙縮脲法（Biuret法）和Folin―酚試劑法（Lowry法）的明顯缺點(diǎn)和許多限制，促使科學(xué)家們?nèi)ふ腋玫牡鞍踪|(zhì)溶液測(cè)定的方法。
1976年由Bradford建立的考馬斯亮蘭法（Bradford法），是根據(jù)蛋白質(zhì)與染料相結(jié)合的原理設(shè)計(jì)的。這種蛋白質(zhì)測(cè)定法具有超過(guò)其他幾種方法的突出優(yōu)點(diǎn)，因而正在得到廣泛的應(yīng)用。這一方法是目前靈敏度zui高的蛋白質(zhì)測(cè)定法。
考馬斯亮蘭G-250染料，在酸性溶液中與蛋白質(zhì)結(jié)合，使染料的zui大吸收峰的位置（lmax），由465nm變?yōu)?95nm，溶液的顏色也由棕黑色變?yōu)樘m色。經(jīng)研究認(rèn)為，染料主要是與蛋白質(zhì)中的堿性氨基酸（特別是精氨酸）和芳香族氨基酸殘基相結(jié)合。
在595nm下測(cè)定的吸光度值A(chǔ)595，與蛋白質(zhì)濃度成正比。
Bradford法的突出優(yōu)點(diǎn)是：
（1）靈敏度高，據(jù)估計(jì)比Lowry法約高四倍，其zui低蛋白質(zhì)檢測(cè)量可達(dá)1mg。這是因?yàn)榈鞍踪|(zhì)與染料結(jié)合后產(chǎn)生的顏色變化很大，蛋白質(zhì)－染料復(fù)合物有更高的消光系數(shù)，因而光吸收值隨蛋白質(zhì)濃度的變化比Lowry法要大的多。
（2）測(cè)定快速、簡(jiǎn)便，只需加一種試劑。完成一個(gè)樣品的測(cè)定，只需要5分鐘左右。由于染料與蛋白質(zhì)結(jié)合的過(guò)程，大約只要2分鐘即可完成，其顏色可以在1小時(shí)內(nèi)保持穩(wěn)定，且在5分鐘至20分鐘之間，顏色的穩(wěn)定性。因而*不用像Lowry法那樣費(fèi)時(shí)和嚴(yán)格地控制時(shí)間。
（3）干擾物質(zhì)少。如干擾Lowry法的K+、Na+、Mg2+離子、Tris緩沖液、糖和蔗糖、甘油、巰基乙醇、EDTA等均不干擾此測(cè)定法。

CD30(Ki-1抗原)鼠單克隆抗體

我司還提供其它進(jìn)口或國(guó)產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲(chóng)病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測(cè)、食品安全檢測(cè)等試劑盒以及日本生研細(xì)菌分型診斷血清、德國(guó)SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

想了解更多的產(chǎn)品及服務(wù)請(qǐng)掃描下方二維碼：

【公司名稱(chēng)】 廣州健侖生物科技有限公司
【市場(chǎng)部】    楊永漢

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【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

(A) experimental principle
The obvious shortcomings and many limitations of the Biuret and Folin-Phenol methods (Lowry's) have prompted scientists to find ways to better determine protein solutions.
The Bradford method, established by Bradford in 1976, is based on the combination of protein and dye. This protein assay has many outstanding advantages over several other methods and is therefore being widely used. This method is currently the most sensitive protein assay.
Coomassie brilliant blue G-250 dye, combined with the protein in acidic solution, so that the dye maximum absorption peak position (lmax) from 465nm to 595nm, the solution color from brown to blue. The study suggests that the dye is mainly combined with the basic amino acids in the protein (especially arginine) and aromatic amino acid residues.
The absorbance value A595 measured at 595 nm is proportional to the protein concentration.
The outstanding advantages of the Bradford method are:
(1) High sensitivity, which is estimated to be four times higher than the Lowry method, with a minimum protein detection of 1 mg. This is because the color produced by the binding of the protein to the dye changes greatly, and the protein-dye complex has a higher extinction coefficient, so that the light absorption value changes more strongly with the protein concentration than the Lowry method.
(2) Determination of fast, simple, just add a reagent. To complete a sample determination, only about 5 minutes. Due to the dye-protein binding process, it takes only about 2 minutes to complete and its color can be stable in less than 1 hour with the best color stability between 5 minutes and 20 minutes. Thus there is absoluy no time and strict time control required by the Lowry method.
(3) less interfering substances. Such interference with the Lowry method K +, Na +, Mg2 + ions, Tris buffer, sugar and sucrose, glycerol, mercaptoethanol, EDTA, etc. do not interfere with this assay.