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CarboPac PA200碳水化合物色譜柱
對于高分辨率測繪和糖蛋白釋放的寡糖的分析，使用的Thermo Scientific戴安CARBOPACPA200分析及保護(hù)柱提供的敏感性和特異性檢測。脈沖安培檢測（PAD）相結(jié)合，此陰離子交換柱提供無需使用衍生快速，重復(fù)分離。疏水性聚合物，薄殼型陰離子交換樹脂填充材料是在pH值0-14的范圍內(nèi)保持穩(wěn)定。這種*的pH值穩(wěn)定性允許使用有利于碳水化合物的陽極氧化的金電極洗脫液組成的。
CarboPac PA200碳水化合物色譜柱
訂貨信息：
Dionex CarboPac PA200 碳水化合物分析柱
| 分析柱 | |
| CarboPac PA200 Analytical Column (3 x 250 mm) | 062896 |
| 保護(hù)柱 | |
| CarboPac PA200 Guard Column (3 x 50 mm) | 062895 |
Fast, pH-Stable Separation of Oligosaccharides
A variety of HPLC approaches are proposed for characterizing oligosaccharides, but many prove inadequate for separating complex mixtures and are limited by a lack of specificity and high limits of detection. Dionex has developed an improved HPLC technique for carbohydrate analysis based on anion-exchange chromatography coupled with pulsed amperometric detection. This technique provides high-resolution separations with sensitive and specific detection, without derivatization.
Sensitive, direct detection with no derivatization, using pulsed amperometric detection
Very high-efficiency separations
Separations based on size, charge, degree of branching, and linkage isomerism
Low flow rate saves on eluent consumption
Does not require derivatization
CarboPac? PA200 columns are packed with a hydrophobic, polymeric, pellicular anion-exchange resin stable over the range of pH 0–14. The unique pH-stability of the packing material allows the use of eluent compositions that are conducive to anodic oxidation of carbohydrates at gold electrodes.
| CarboPac PA200 Column for High-Resolution Oligosaccharide Separations Specifications | |
|---|---|
| Dimensions | CarboPac? PA200 Analytical Column: 3 × 250 mm CarboPac PA200 Guard Column: 3 × 50 mm |
| Maximum operating pressure | 4000 psi (27.9 MPa) |
| Temperature range | 6–60 °C Recommended operating temperature: controlled ambient |
| Typical operating conditions | 2700 psi at 0.5 mL/min (analytical and guard columns) Recommended flow rate: 0.5 mL/min |
| Mobile phase compatibility | pH 0–14; 100% compatible with common organic solvents. Ionic form eluents: sodium acetate and sodium hydroxide only. |
| Resin composition | 5.5 μm-diameter ethylvinylbenzene/divinylbenzene substrate (55% cross-linked) agglomerated with 43 nm MicroBead? quaternary amine functionalized latex (6% crosslinked) |
| Anion-exchange capacity | Approximately 35 μeq/column (3 × 250 mm analytical column) |
| Column construction | PEEK? with 10-32 threaded ferrule-style end fittings. All components are nonmetallic |
