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ThermoFisher	AM1340	mMESSAGE mMACHINE® SP6 Transcription Kit
ThermoFisher	AM1344	mMESSAGE mMACHINE® T7 Transcription Kit
ThermoFisher	AM1348	mMESSAGE mMACHINE® T3 Transcription Kit

invitrogen/AM1348/AM1344/AM1340現貨

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描述

mMESSAGE mMACHINE® kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5' end. mMESSAGE mMACHINE® kit reactions include cap analog [m7G(5')ppp(5')G] in an ultra high-yield transcription reaction. The cap analog is incorporated only as the first or 5' terminal G of the transcript because its structure precludes its incorporation at any other position in the RNA molecule.

How mMESSAGE mMACHINE® kits work
mMESSAGE mMACHINE® kits have a simplified reaction format in which all four ribonucleotides and cap analog are mixed in a single solution. The cap analog:GTP ratio of this solution is 4:1, which is optimal for maximizing both RNA yield and the proportion of capped transcripts. mMESSAGE mMACHINE® kits are ideal for the routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection, and other applications. The high yields are achieved by optimizing reaction conditions for RNA synthesis in the presence of high nucleotide concentrations. In addition, the RNA synthesized is protected from degradation by any contaminating ribonucleases that may be present with RNase inhibitor—a component of the Enzyme Mix.

Using mMESSAGE mMACHINE® Kits
In a 20 µL reaction during a 2 hour incubation, mMESSAGE mMACHINE® High Yield Capped RNA Transcription will yield large mass amounts of capped RNA. Up to 10–50 times the yield obtained with conventional in vitro transcription reactions. The ratio of cap analog to GTP has been optimized to allow the best compromise between yield (15–35 µg) and proportion of transcripts that are capped (80%). mMESSAGE mMACHINE® Kits also contain a LiCl precipitation solution that is efficient for separating proteins and unincorporated nucleotides (including free cap analog) from the capped RNA, allowing an increased efficiency of translation. mMESSAGE mMACHINE® kits are only optimized for use with the polymerases included in the kit. Using a different polymerase may result in low yields. The mMESSAGE mMACHINE® kits contain all the buffers and reagents necessary for 25 transcription reactions. Using the control template supplied with the kits (Xenopus elongation factor 1α, pTRI Xef), each mMESSAGE mMACHINE® reaction will yield approximay 20–30 µg of RNA using T3 or T7 RNA polymerase, or about 15–25 µg RNA using SP6 RNA polymerase.

For Research Use Only. Not for use in diagnostic procedures.

invitrogen/AM1348/AM1344/AM1340現貨

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螞蟻淘(ebiomall）是一家生命科學領域的垂直電商平臺，公司專注生物醫(yī)學科研用品的  導購和品牌推廣，為海外廠方與國內代理經銷商搭建跨境貿易系統(tǒng)和交流平臺。
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螞蟻淘常見平行進口產品有：
Thermofisher旗下Gibco培養(yǎng)系列、ABI細胞計數儀及PCR儀、Invitrogen分子生物學試劑及Qubit3.0、Ambion的RNA相關試劑、Molecular probes試劑、Pierce蛋白相關試劑盒、Nanodrop One/OneC；Parafilm封口膜；Viskase透析袋；Sigma-Aldrich試劑；AccuStandard 標準品；Millipore抗體；Glen Research 核酸合成試劑；Bio-Rad電泳儀及PCR儀；Nanocs的PEG聚合物；  Chromotek羊駝抗體；   Polysciences轉染試劑；  Echelon-inc 脂類研究試劑盒；   R&D Systems抗體及Elisa試劑盒； Jackson二抗及封閉血清； Biotium 熒光染料；NEB內切酶及NGS相關產品；  Athens Research & Technology人類蛋白及抗血清；  Illumina二代測試試劑盒； Tocris 小分子試劑； TwistDx恒溫擴增試劑盒（RPA）； Enzymatics二代測序酶；Novus抗體及Elisa試劑盒；AG scientific潮霉素B、G-418；Plantmedia植物培養(yǎng)系列及抗體；Agilent 2100生物分析儀；Biolog耗材；Biomatik的Elisa試劑盒；Covaris基因打斷儀；CST抗體；Dharmacon的siRNA產品。