国产精品视频一区二区三区四,亚洲av美洲av综合av,99国内精品久久久久久久,欧美电影一区二区三区电影

上海徠同生物科技有限公司

zeta life轉(zhuǎn)染犬/狗脂肪間充質(zhì)干細(xì)胞(ADMSCs)

時(shí)間:2022-4-12閱讀:188
分享:

 

一、轉(zhuǎn)染犬/狗脂肪間充質(zhì)干細(xì)胞(ADMSCs)siRNA基因沉默 簡(jiǎn)述

熒光素標(biāo)記的siRNA(小干擾 RNA)每個(gè)基因設(shè)計(jì)有三個(gè)siRNA,使用美國(guó)Zeta Life公司Advanced DNA RNA轉(zhuǎn)染試劑(Zeta Life,Menlo Park,CA,United States)用siRNA轉(zhuǎn)染犬/狗脂肪間充質(zhì)干細(xì)胞(ADMSCs),RT-qPCR用于檢測(cè)siRNA 的沉默效率,并選擇沉默效率zui高的一種,在整個(gè)轉(zhuǎn)染過(guò)程中,該基因每7天轉(zhuǎn)染一次以保持沉默。

二、轉(zhuǎn)染犬/狗脂肪間充質(zhì)干細(xì)胞(ADMSCs)基因過(guò)表達(dá) 簡(jiǎn)述

使用美國(guó)Zeta Life公司Advanced DNA RNA轉(zhuǎn)染試劑(Zeta Life,Menlo Park,CA,USA)轉(zhuǎn)染AAV-293細(xì)胞收集重組腺病毒顆粒并感染犬/狗脂肪間充質(zhì)干細(xì)胞(ADMSCs),用 RT-qPCR和蛋白質(zhì)印跡檢測(cè)目的基因的表達(dá)。

三、培養(yǎng)條件 簡(jiǎn)述

脂肪間充質(zhì)干細(xì)胞(ADMSCs),使用美國(guó)Zeta Life公司z7010FBS-500胎牛血清培養(yǎng)。

四、Adenovirus-Mediated Gene Overexpression原文分享

The cDNA sequences for genes were synthesized by Wuhan Gene Create Biological Engineering Co., Ltd. (Wuhan, China). The cDNA for each gene was PCR-amplifified and inserted into pAdTrack-CMV previously digested with BglII/HindIII using the In-Fusion HD cloning kit (Takara, Japan) (Sleight et al., 2010). The resulting plasmid was linearized with PmeI, and then homologous recombination was performed with pAdeasy-1 in E. coli BJ5183. The recombinant plasmid with PacI linearization was used to transfect AAV-293 cells to

produce recombinant adenovirus particles using the Advanced DNA RNA transfection reagent (Zeta Life, Menlo Park, CA, United States). RT-qPCR (Nolan et al., 2006) and Western-blot (following the Elabscience Western blot detection kit operation manual) were used to detect the expression of target gene. The canine ADMSCs were transfected with the recombinant adenovirus particles.

五、siRNA-Mediated Gene Silencing原文分享

Fluorescein-labeled siRNA . Each gene was designed with three siRNAs. In the process of transdifffferentiation, the canine ADMSCs were transfected with siRNAs using the Advanced DNA RNA transfection reagent (Zeta Life, Menlo Park, CA, United States). RT-qPCR (Nolan et al., 2006) was used to detect the silencing effiffifficiency of siRNAs and the one with the highest silencing effiffifficiency was selected. The gene was silenced every 7 days throughout the transdifffferentiation to keep it silent.

六、美國(guó)Zeta Life 公司與美國(guó)加利福尼亞大學(xué)舊金山校區(qū)聯(lián)合開(kāi)發(fā)用于哺乳動(dòng)物細(xì)胞、活體動(dòng)物轉(zhuǎn)染的 Advanced DNA RNA 第三代多肽小分子轉(zhuǎn)染試劑,此技術(shù)成為新的蛋白功能、免疫細(xì)胞及干細(xì)胞治療、研發(fā)及生產(chǎn)的主要關(guān)鍵技術(shù)之一。

七、產(chǎn)品信息

會(huì)員登錄

×

請(qǐng)輸入賬號(hào)

請(qǐng)輸入密碼

=

請(qǐng)輸驗(yàn)證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~

以上信息由企業(yè)自行提供,信息內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé),化工儀器網(wǎng)對(duì)此不承擔(dān)任何保證責(zé)任。

溫馨提示:為規(guī)避購(gòu)買風(fēng)險(xiǎn),建議您在購(gòu)買產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。

撥打電話
在線留言
沈丘县| 德钦县| 吉水县| 本溪| 镇安县| 聂荣县| 贵阳市| 郑州市| 霍林郭勒市| 普格县| 双牌县| 崇左市| 临猗县| 永川市| 北辰区| 广安市| 营口市| 习水县| 三都| 攀枝花市| 甘德县| 福鼎市| 宜昌市| 称多县| 昌平区| 鄂托克旗| 西丰县| 东乡县| 龙川县| 濮阳市| 武义县| 鄂托克旗| 都兰县| 开阳县| 巢湖市| 乌鲁木齐市| 三台县| 赤峰市| 肥乡县| 菏泽市| 体育|