国产精品视频一区二区三区四,亚洲av美洲av综合av,99国内精品久久久久久久,欧美电影一区二区三区电影

上海徠同生物科技有限公司

線性PEI轉(zhuǎn)染試劑速溶型MW40000的產(chǎn)品特點介紹

時間:2022-11-17閱讀:175
分享:

線性PEI轉(zhuǎn)染試劑(速溶型)MW40000

英文名Polyethylenimine Linear(PEI) MW40000(rapid lysis)貨號KE1098保存條件室溫

線性PEI轉(zhuǎn)染試劑是一種陽離子聚合物,分子量40000,它能與核酸形成復(fù)合物,并使該復(fù)合物進入哺乳動物細胞。線性PEI轉(zhuǎn)染試劑廣泛適用于常見細胞系,如 HEK-293、HEK293T、 Hep G2、Hela、CHO-K1、COS-1、COS-7、NIH/3T3 和 Sf9 等。該試劑即使在有血清存在的情況下,它仍然能高效的將核酸導(dǎo)入細胞。我公司提供的線性PEI轉(zhuǎn)染試劑具有如下優(yōu)點:*的轉(zhuǎn)染效率 , 重組蛋白的高表達水平,與含血清的培養(yǎng)基相兼容, 低細胞毒性,易于操作。

本產(chǎn)品為速溶型,溶解迅速,配制方便。

說明書

Transfection Reagent Preparation and Storage Recommendations

Transfection Reagent Preparation (1 mg/mL)

Materials

1.1g PEI MAX 40K (kingmorn Catalog#KE1098)

2.1L Milli-Q® water, water for injection (WFI), or comparable biological-grade water

3.1N Sodium Hydroxide, USP

4.Sterile 25 mL plastic pipette

5.0.1µm, 0.2µm, or 0.22µm PES vacuum sterile-filter unit

6.Sterile HDPE or polypropylene storage vials

Equipment

1.1L glass beaker

2.PTFE-coated stir bar

3.Magnetic stir plate

4.1L glass graduated cylinder

5.pH meter

6.Pipette controller

7.Vacuum pump with tubing

Method

1.In 1L glass beaker, suspend 1g of PEI MAX 40K in 900 mL water.

2.Add PTFE-coated stir bar to 1L glass beaker and set stirring to produce small vortex.

3.Wait for PEI MAX 40K to completely dissolve. This typically takes less than 5 minutes.

4.Use 25 mL plastic pipette to add 1N sodium hydroxide dropwise to 1L glass beaker until pH is 6.90 to 7.10.

5.If pH accidently exceeds 7.10, use 1N hydrochloric acid and a new 25 mL plastic pipette to lower pH back to 6.90 to 7.10.

6.Decant solution from 1L glass beaker to 1L graduated cylinder.

7.Add water to 1L graduated cylinder to adjust final volume to 1L.

8.Sterile-filter solution through vacuum membrane.

9.Aliquot solution as desired.

10.Store aliquots at 4°C

Transfection Solution Storage and Shelf Life

At 4°C in a suitable container, the transfection solution will retain its maximum performance for at least six months. For qualitative work, the solution may be suitable for use for up to a year.


會員登錄

×

請輸入賬號

請輸入密碼

=

請輸驗證碼

收藏該商鋪

X
該信息已收藏!
標簽:
保存成功

(空格分隔,最多3個,單個標簽最多10個字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時間回復(fù)您~

以上信息由企業(yè)自行提供,信息內(nèi)容的真實性、準確性和合法性由相關(guān)企業(yè)負責(zé),化工儀器網(wǎng)對此不承擔任何保證責(zé)任。

溫馨提示:為規(guī)避購買風(fēng)險,建議您在購買產(chǎn)品前務(wù)必確認供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。

撥打電話
在線留言
宾阳县| 灯塔市| 祁连县| 荔波县| 德钦县| 陆良县| 永州市| 鹿泉市| 东方市| 凤阳县| 玛纳斯县| 东兴市| 湘西| 新民市| 诏安县| 崇文区| 集贤县| 南康市| 邓州市| 彭山县| 眉山市| 黑水县| 株洲市| 永州市| 丹棱县| 吉首市| 兴国县| 清河县| 临泽县| 太白县| 枝江市| 珲春市| 海城市| 革吉县| 吉水县| 康保县| 榆林市| 阳泉市| 济南市| 临洮县| 四平市|