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當(dāng)前位置:武漢艾美捷科技有限公司>>技術(shù)文章>>中英文說(shuō)明丨艾美捷017-000-121正常驢血清介紹
017-000-121正常驢血清英文說(shuō)明:
Information
Normal serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide, and freeze dried. Normal serum diluted to 5% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific, conserved sequence and/or Fc receptor binding. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.
Usage
Physical State: Freeze-dried solid
Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Preservative: 0.05% Sodium Azide
Suggested Working Concentration or Dilution Range:
For blocking purposes, use 5% (v/v) solution (1:20 dilution from rehydrated volume). Rehydration according to instruction yields 100% serum.
017-000-121正常驢血清(艾美捷)中文說(shuō)明:
正常血清經(jīng)脂質(zhì)提取以提高透明度,用含疊丨氮丨化鈉的磷酸鹽緩沖鹽水(PBS)透析,然后冷凍干燥。強(qiáng)烈推薦在PBS中稀釋至5%(v/v)的正常血清作為阻斷試劑,以減少非特異性、保守序列和/或Fc受體結(jié)合的背景。在添加一級(jí)抗體之前,使用來(lái)自與標(biāo)記抗體相同的宿主的稀釋的正常血清作為單獨(dú)的孵育步驟獲得最佳結(jié)果。
艾美捷017-000-121正常驢血清基本參數(shù):
Host: Donkey
Product:
Conjugate: Unconjugated
Product Category: Normal Serums and Gamma Globulins
RRID: AB_2337258
注意:
1、017-000-121正常驢血清可應(yīng)用于大多數(shù)免疫學(xué)實(shí)驗(yàn),用作封閉液或空白對(duì)照.
2、凍干粉末形式,保存在2-8°C,準(zhǔn)備用時(shí),根據(jù)指示的體積加入雙蒸水,在2-8°C條件下,未稀釋的液體保質(zhì)期6周,每天準(zhǔn)備新鮮的工作液.如果重溶后,想延長(zhǎng)保存期,將未稀釋的溶液保存在-20°C或者更低,避免反復(fù)凍融.
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