This antibody reacts against the synthetic thymidine analog BrdU, which when injected into an animal is incorporated during the S phase of the cell cycle into newly synthesized DNA strands of actively proliferating cells. The number of cells that survive can be measured by immunocytochemistry. As an outstanding tool to assess the proliferation state of a group of cells, it has become vital to drug discovery research, especially with cancer therapeutics and ascertaining the health of cells during ADME/Tox studies. BrdU Antibody-W is a polyclonal raised in rabbit against BrdU conjugated to bovine serum albumin (BSA). Our antibody has

been used for immunocytochemistry in rat brain tissue. It will be sold with filter paper absorbed with BrdU for blocking. This allows the researcher to confirm the specific sign

艾美捷BrdU抗體是一種多克隆抗體在兔體內(nèi)針對結(jié)合牛血清白蛋白（BSA）的BrdU培養(yǎng)。Fluorochrome的抗體用于大鼠腦組織的免疫細(xì)胞化學(xué)。

BrdU抗體（FC60001）基本參數(shù)：

中文名稱：BrdU抗體

英文名字：BrdU Antibody -W

cat#：FC60001

規(guī)格：200ul

應(yīng)用類型：IHC

免疫原：BrdU結(jié)合牛血清白蛋白（BSA）

來源宿主：兔

反應(yīng)性：大鼠

保存建議：小瓶含有約200μl 1/100稀釋液。 BrdU抗體-W可在BSA稀釋劑（50 mM KPBS、0.4%Triton、1%BSA、1%NGS）中進(jìn)一步稀釋至至少1/30，000。n抗體應(yīng)在-20 ℃下儲存。 您將收到解凍的抗體。 除非你打算在48小時內(nèi)使用，否則請重新冷凍。 避免多次重新冷凍抗體。n還包括吸附BrdU的濾紙，用于阻塞。 可向試管中加入500μl稀釋抗體，以獲得10 μM的組織塊。 應(yīng)用于組織前，在室溫（RT）下孵育至少1小時。 該抗體特異性阻斷BrdU，不阻斷濾紙上吸附的其他肽。

BrdU抗體（FC60001）特異性：

通過進(jìn)行阻斷研究確定了特異性。從將先前注射BrdU的成年大鼠的海馬與單獨(dú)使用BrdU Antibody-W（A-60X）或與BrdU預(yù)孵育的BrdU Antibody-W一起使用（B-60X）。注意A中的標(biāo)記和B中的標(biāo)記缺失，表明抗體的特異性舞臺調(diào)度。

A.齒狀回BrdU陽性細(xì)胞（60X）成年大鼠注射BrdU。在共焦顯微鏡上拍攝的照片

B.齒狀回B.BrdU阻滯

文獻(xiàn)：

Garcia-Fuster et al, in preparation: Individual differences in adult hippocampal neurogensis following experimenter administration of cocaine.