国产精品视频一区二区三区四,亚洲av美洲av综合av,99国内精品久久久久久久,欧美电影一区二区三区电影

靶點(diǎn)科技(北京)有限公司

Kerafast Anti-DNA-RNA抗體ENH001簡(jiǎn)介

時(shí)間:2022-7-11閱讀:3463

 

產(chǎn)品介紹:

Kerafast的貨號(hào):ENH001,Anti-DNA-RNA Hybrid [S9.6] Antibody,這種小鼠單克隆抗體是針對(duì) ΦX174 噬菌體衍生的合成 DNA-RNA 抗原生成的,可識(shí)別各種長(zhǎng)度的 RNA-DNA 雜合體。


特色:

可用于檢測(cè) R 環(huán)

對(duì) DNA-RNA 雜交體的高特異性和親和力

不與單鏈 DNA 或雙鏈 DNA 發(fā)生交叉反應(yīng)

對(duì)于富含 AU 的雙鏈 RNA,觀察到了輕微的交叉反應(yīng)(約 5 倍以下)。

長(zhǎng)度為 8、10、15 和 23 個(gè)堿基對(duì)的雜交體顯示出高親和力結(jié)合


DNA-RNA 雜合體是真核細(xì)胞中的一種自然現(xiàn)象,這些雜合體的水平在具有高轉(zhuǎn)錄活性的位點(diǎn)增加,例如在轉(zhuǎn)錄起始、抑制和延伸期間。由于 RNA-DNA 雜合體會(huì)影響基因組的不穩(wěn)定性,因此 S9.6 抗體是一種有用的試劑,可幫助研究在 DNA 復(fù)制或其他細(xì)胞過(guò)程中由這些雜合體形成的 R 環(huán)和損傷的后果。此外,S9.6 抗體可有效識(shí)別用于微陣列研究的 RNA-DNA 雜交。

 

This mouse monoclonal antibody was generated against a ΦX174 bacteriophage-derived synthetic DNA–RNA antigen and recognizes RNA-DNA hybrids of various lengths.

Highlights:

* Useful in the detection of R-loops

* High specificity and affinity for DNA-RNA hybrids

* Does NOT cross-react with single-stranded DNA or double-stranded DNA

* Minor cross-reaction (~5-fold less) has been observed for AU-rich double-stranded RNA.

* High affinity binding shown for hybrids of 8, 10, 15, and 23 base pairs in length


 


產(chǎn)品詳情:

Product Type: Antibody
Name: Anti-DNA-RNA Hybrid [S9.6]
Antigen: S9.6 ΦX174 bacteriophage-derived synthetic DNA–RNA antigen
Isotype: Rabbit IgG
Fusion Tag(s): Mouse Fab version contains His-tag
Clone Name: S9.6
Reactivity: High specificity and affinity for DNA/RNA hybrids and other A-form nucleic acid hybrids
Immunogen: ΦX174 bacteriophage-derived synthetic DNA/RNA
Purification Method: Protein A/G
Buffer: ENHOO1: PBS, 0.05% (w/v) Sodium Azide
Ab01137- : PBS with 0.02% Proclin 300
Tested Applications:

Dot Blot Analysis: 0.2 µg/mL.
Affinity Binding Assay: Clone S9.6 bound the DNA-RNA heteropolymer and poly(I)-poly(dC) equally, but 100-fold higher levels of poly(A)-poly(dT) were required to achieve a similar degree of binding. Single-stranded DNA, double-stranded DNA and RNA, and ribosomal RNA were not bound by clone S9.6 (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected increased DNA RNA hybrids at four actively transcribed genes upon shRNA-mediated knockdown of BRCA1 or BRCA2, but not PCID2 or RAD51 in HeLa cells (Bhatia, V., et al. (2014). Nature. 511(7509):362-365).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected R-loops formed over beta-actin gene using HeLa chromatin preparation. RNase H treatment of the chromatin preparation prevented clone S9.6 from immunoprecipitating target chromatin fragments (Skourti-Stathaki, K., et al. (2011). Mol. Cell. 42(6):794-805).
Chromatin Immunoprecipitation-sequencing (ChIP-seq) Analysis: A representative lot detected genome-wide distribution of DNA-RNA hybrids in budding yeast by ChIP-seq analysis (El Hage, A., et al. (2014). PLoS Genet. 10(10):e1004716).
Immunocytochemistry Analysis: Representative lots immunolocalized nuclear R loops by fluorescent immunocytochemistry staining of methanol-fixed H1 human embryonic stem cells (hESCs) and formaldehyde-fixed HeLa cells (Bhatia, V., et al. (2014). Nature. 511(7509):362-365; Ginno, P.A., et al. (2012). Mol. Cell. 45(6):814-825).
Immunoprecipitation Analysis: A representative lot immunoprecipitated in vitro transcribed R-loop substrate (DNA-RNA hybrid), but not doouble-stranded DNA (dsDNA) (Ginno, P.A., et al. (2012). Mol. Cell. 45(6):814-825).

 


參考文獻(xiàn):

1. Dutrow N, Nix DA, Holt D, Milash B, Dalley B, Westbroek E, Parnell TJ, Cairns BR. Dynamic transcriptome of Schizosaccharomyces pombe shown by RNA-DNA hybrid mapping. Nat Genet. 2008 Aug;40(8):977-86.

2. Bhatia V, Barroso SI, García-Rubio ML, Tumini E, Herrera-Moyano E, Aguilera A. BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2. Nature. 2014 Jul 17;511(7509):362-5.

3. Lim J, Giri PK, Kazadi D, Laffleur B, Zhang W, Grinstein V, Pefanis E, Brown LM, Ladewig E, Martin O, Chen Y, Rabadan R, Boyer F, Rothschild G, Cogné M, Pinaud E, Deng H, Basu U. Nuclear Proximity of Mtr4 to RNA Exosome Restricts DNA Mutational Asymmetry. Cell. 2017 Apr 20;169(3):523-537.e15.

4. Lang KS, Hall AN, Merrikh CN, Ragheb M, Tabakh H, Pollock AJ, Woodward JJ, Dreifus JE, Merrikh H. Replication-Transcription Conflicts Generate R-Loops that Orchestrate Bacterial Stress Survival and Pathogenesis. Cell. 2017 Aug 10;170(4):787-799.e18.

5. De Cecco M, Ito T, Petrashen AP, Elias AE, Skvir NJ, Criscione SW, Caligiana A, Brocculi G, Adney EM, Boeke JD, Le O, Beauséjour C, Ambati J, Ambati K, Simon M, Seluanov A, Gorbunova V, Slagboom PE, Helfand SL, Neretti N, Sedivy JM. L1 drives IFN in senescent cells and promotes age-associated inflammation. Nature. 2019 Feb;566(7742):73-78.

6. Herold S, Kalb J, Büchel G, Ade CP, Baluapuri A, Xu J, Koster J, Solvie D, Carstensen A, Klotz C, Rodewald S, Schülein-Völk C, Dobbelstein M, Wolf E, Molenaar J, Versteeg R, Walz S, Eilers M. Recruitment of BRCA1 limits MYCN-driven accumulation of stalled RNA polymerase. Nature. 2019 Mar;567(7749):545-549

7. Sanz LA, Chédin F. High-resolution, strand-specific R-loop mapping via S9.6-based DNA-RNA immunoprecipitation and high-throughput sequencing. Nat Protoc. 2019 Jun;14(6):1734-1755.

8. Graf M, Bonetti D, Lockhart A, Serhal K, Kellner V, Maicher A, Jolivet P, Teixeira MT, Luke B. Telomere Length Determines TERRA and R-Loop Regulation through the Cell Cycle. Cell. 2017 Jun 29;170(1):72-85.e14.

9. Gorthi A, Romero JC, Loranc E, Cao L, Lawrence LA, Goodale E, Iniguez AB, Bernard X, Masamsetti VP, Roston S, Lawlor ER, Toretsky JA, Stegmaier K, Lessnick SL, Chen Y, Bishop AJR. EWS-FLI1 increases transcription to cause R-loops and block BRCA1 repair in Ewing sarcoma. Nature. 2018 Mar 15;555(7696):387-391.

訂購(gòu)詳詢靶點(diǎn)科技。靶點(diǎn)科技現(xiàn)貨庫(kù)存。

會(huì)員登錄

X

請(qǐng)輸入賬號(hào)

請(qǐng)輸入密碼

=

請(qǐng)輸驗(yàn)證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~

以上信息由企業(yè)自行提供,信息內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé),化工儀器網(wǎng)對(duì)此不承擔(dān)任何保證責(zé)任。

溫馨提示:為規(guī)避購(gòu)買(mǎi)風(fēng)險(xiǎn),建議您在購(gòu)買(mǎi)產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。

撥打電話
在線留言
扶绥县| 伊金霍洛旗| 镇坪县| 孟州市| 岑溪市| 潼南县| 濮阳县| 鄂尔多斯市| 舒城县| 旬邑县| 多伦县| 清水县| 九台市| 剑川县| 嘉义市| 普兰店市| 宜昌市| 长宁县| 通渭县| 伊通| 湘潭县| 资阳市| 凤冈县| 嘉黎县| 若尔盖县| 江油市| 商河县| 瓦房店市| 博客| 庄河市| 离岛区| 肥城市| 新民市| 江永县| 河北省| 嘉祥县| 丹凤县| 阜南县| 平塘县| 博湖县| 静安区|