国产精品视频一区二区三区四,亚洲av美洲av综合av,99国内精品久久久久久久,欧美电影一区二区三区电影

靶點(diǎn)科技(北京)有限公司

小鼠脾臟細(xì)胞分離Protocol Mouse Spleen Cell Isolation Protocol

時(shí)間:2024-6-20閱讀:812

脾臟是造血、紅細(xì)胞清除和免疫功能的場所,因此是細(xì)胞質(zhì)控的良好來源。它可以過濾細(xì)胞碎片、病原體和不規(guī)則細(xì)胞。它是紅細(xì)胞和白細(xì)胞以及幾種免疫細(xì)胞亞型的來源,包括粒細(xì)胞、單核細(xì)胞、巨噬細(xì)胞、樹突狀細(xì)胞 (DC)、NK 細(xì)胞、T 細(xì)胞和 B 細(xì)胞。


小鼠脾臟細(xì)胞分離Protocol Mouse Spleen Cell Isolation Protocol

操作步驟

Perform steps 1–7 at room temperature and steps 8–12 on ice with cold buffers.

1. Obtain fresh whole mouse spleen.

2. Place mouse spleen into petri dish with 5 mL HBSS (Hank’s balanced salt solution) buffer.

3. Carefully mince the spleen into small pieces (~0.2 cm2) with a razor or scalpel blade.

4. For preparation of myeloid cells (continue to step 5 for crude preparation): Incubate the excised spleen pieces for 20-30 min at 37°C with 5 mL of HBSS solution containing Collagenase IV (100 U/mL), DNase I (20 U/mL), and 1% FBS.

5. Add EDTA to the solution to a concentration of 1 mM for 5 minutes at room temperature to stop the enzymatic reaction.

6. Place cell strainer over a 50 mL conical tube.

7. With a disposable transfer pipette, transfer the excised spleen into the cell strainer.

8. With the plunger end of a syringe, mash or press the spleen through the strainer. Add 5–10 mL PBS if necessary.

9. Wash the cells through the strainer with excess PBS. Repeat step 5 and 6, if needed.

10. Centrifuge the cells at 400–600 x g for 5 minutes at 4 °C; discard the supernatant.

11. Resuspend the cell pellet in 2–5 mL of cold 1x RBC Lysis buffer.

12. Incubate the suspension for 5 minutes on ice.

13. Wash the cell suspension with 10–20 mL cold PBS.

14. Centrifuge the cells at 400–600 x g for 5 minutes at 4 °C; discard the supernatant.

15. Resuspend the cell pellet in PBS at 2–3 x 106 cells/mL.




會(huì)員登錄

X

請輸入賬號(hào)

請輸入密碼

=

請輸驗(yàn)證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~

以上信息由企業(yè)自行提供,信息內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé),化工儀器網(wǎng)對此不承擔(dān)任何保證責(zé)任。

溫馨提示:為規(guī)避購買風(fēng)險(xiǎn),建議您在購買產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。

撥打電話
在線留言
新余市| 罗田县| 安图县| 雷州市| 边坝县| 容城县| 城口县| 启东市| 青铜峡市| 辛集市| 宁远县| 金山区| 巴里| 广安市| 临城县| 合肥市| 万源市| 噶尔县| 安多县| 淮北市| 东丰县| 琼海市| 定日县| 镇远县| 安岳县| 梧州市| 旺苍县| 邳州市| 连南| 巴塘县| 济宁市| 乐陵市| 饶河县| 永和县| 阳原县| 滨州市| 温宿县| 锡林浩特市| 江陵县| 太湖县| 汶川县|