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人小細(xì)胞肺癌細(xì)胞NCI-H446

種屬	人
別稱	H446; H-446; NCI-446; NCIH446
組織來源	肺，來源于轉(zhuǎn)移部位：胸腔積液
疾病	肋膜滲出癌，小細(xì)胞肺癌
傳代比例/細(xì)胞消化	1:2傳代,浮部分離心收集(1000RPM,5分鐘) ，貼壁部分消化1-2分鐘
培養(yǎng)基配置	RPMI1640培養(yǎng)基；10%胎牛血清；1%雙抗
簡介	NCI-H446細(xì)胞株從一位小細(xì)胞肺癌患者的胸水中建立。細(xì)胞的原始形態(tài)并不具有小細(xì)胞肺癌特征。這個(gè)細(xì)胞株是小細(xì)胞肺癌的生化和形態(tài)學(xué)上的變種，表達(dá)神經(jīng)元的烯醇酶和腦部肌酸激酶同功酶。脫羧酶、蠶素、抗利尿激素、催產(chǎn)素或胃泌激素釋放肽未達(dá)到可檢測水平。C-myc DNA序列擴(kuò)增約20倍，c-myc RNA比正常細(xì)胞增加 15倍。最初傳代培養(yǎng)基用添加10 nM , 0.005 mg/ml 胰島素, 0.01 mg/ml 鐵傳遞蛋白, 10 nM 17- beta-雌二醇,30 nM 的RPMI 1640, 95% ，胎牛血清, 5%
形態(tài)	上皮細(xì)胞樣
生長特征	貼壁，懸浮混合生長
致瘤性	Yes, in nude mice (The cells form transplantable tumors with non-typical SCLC histology).
倍增時(shí)間	每周 2 至 3 次
保藏機(jī)構(gòu)	ATCC; HTB-171
備注	該細(xì)胞為半懸浮和半貼壁細(xì)胞，懸浮細(xì)胞離心收集，貼壁細(xì)胞消化處理

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immunotherapy from the perspective of somatic evolution and discuss the basic rules of resistance to immunotherapies as dictated by evolution.The main breakthrough in tumor immunotherapy was the discovery of immune checkpoint (IC) proteins, which act as a potent