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人膀胱移行細(xì)胞癌UM-UC-3

種屬	人
別稱	UMUC-3; UM-UC3; UMUC3; UC-3; University of Michigan-Urothelial Carcinoma-3
組織來源	膀胱
疾病	移行細(xì)胞癌
傳代比例/細(xì)胞消化	1:2傳代，消化2-3分鐘
培養(yǎng)基配置	MEM培養(yǎng)基；10%胎牛血清；1%雙抗
簡介	Derived from a urinary bladder transitional cell carcinoma from a human male. These cells are tumourigenic in nude mice when 10E7 cells are inoculated subcutaneously. Hypertriploid. The modal chromosome number was 80, occurring in 42% of cells. Cells with 78 chromosomes also occurred at a high frequency. The rate of cells with higher ploidies was 2.5%. There were 30 or more marker chromosomes in each cell. They included der(1)t (1;?) (p32;?), ?t(1p5p), i(3q), t(7q14q), ?t(2p3p) ａnd others. The X and N3 had single copy per cell, and others were generally two or three copies per cell.
形態(tài)	上皮細(xì)胞樣
生長特征	貼壁生長
倍增時(shí)間	每周 2 至 3 次
致瘤性	Yes, Tumors developed within 21 days at 99% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.
STR	Amelogenin: X CSF1PO: 10,11 D13S317: 8 D16S539: 8,9 D5S818: 12 D7S820: 8,9 THO1: 6,9 TPOX: 10 vWA: 17
培養(yǎng)條件	氣相：空氣，95% ；二氧化碳，5%。溫度：37攝氏度，培養(yǎng)箱濕度為70%-80%。
凍存條件	凍存液：90%FBS ，DMSO 10%, 或使用非程序凍存液：貨號(hào)JY-H040
保藏機(jī)構(gòu)	ATCC; CRL-1749
產(chǎn)品使用	于科學(xué)研究，不可作為動(dòng)物或人類疾病的治療產(chǎn)品使用。

Compared with the control group，the expression levels of Afamin and DBP in serum of the POI group were significantly increased (P＜0．05) ; the expression levels of serum Afamin and DBP were positively correlated with FSH and E2 (P＜ 0．05) ，but negatively correlated with AMH (P＜0. 05) ;