The PARP2 Homogeneous Assay Kit is designed to measure PARP2 (poly-ADP-ribose) polymerase 2) activity for screening and profiling applications. The PARP2 Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with biotinylated histone substrate, recombinant antibody (ADP-ribose binding reagent 1), PP-01 assay buffer, and purified recombinant human PARP2 (amino acids 2-583) for 384 enzyme reactions. The PARP2 Homogeneous Assay Kit takes advantage of a highly specific antibody that recognizes the PARylated form of the substrate.

Figure 1. PARP2 Homogenous Assay Kit schematic. First, a sample containing PARP2 enzyme is incubated with biotinylated substrate and NAD, followed by addition of acceptor beads and primary antibody. Finally, donor beads are added, and Alpha-counts can be counted. Alpha-counts correlate with PARP2 activity.