The SUV4-20H1 Chemiluminescent Assay Kit is designed to measure SUV4-20H1 activity for screening and profiling applications. The key to the SUV4-20H1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes a methylated residue of Histone H4. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.