The G9a Chemiluminescent Assay Kit is designed to measure G9a activity for screening and profiling applications. This kit comes in a convenient format, with a plate precoated with histone H3 peptide substrate, recombinant human G9a enzyme (amino acids 785-1210), high specific antibody against the methylated lysine residue of Histone H3, secondary HRP-labeled antibody, S-adenosylmethionine and Methyltransferase Assay Buffer for 100 enzyme reactions.

Figure 1: G9a Chemiluminescent Assay Kit workflow diagram. First, S-adenosylmethionine is incubated with the assay buffer, G9a enzyme, and the inhibitor of choice for one hour in a histone-precoated plate. This is followed by the addition of a primary antibody against Lys(Me). Addition of a chemiluminescent HRP substrate provides a luminescence signal, which directly correlates with the activity of G9a.

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