The EZH2/EED/SUZ12/RbAp48/AEBP2 direct activity assay kit (EZH2 Chemiluminescence Assay Kit) is a chemiluminescence three step kit designed to measure activity of the EZH2 (enhancer of zeste homolog 2) complex (EZH2/EED/SUZ12/RbAp48/AEBP) for screening and profiling purposes. The EZH2 Assay Kit comes in a convenient format, with a 96-well plate precoated with histone H3 peptide substrate, an antibody against methylated K27 residue of Histone H3, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified EZH2 complex for 100 enzyme reactions.

EZH2 Activity Assay Kit uses a highly specific antibody that recognizes methylated Histone H3 on K27. In a first step, S-adenosylmethionine is incubated with methyltransferase and sample prepared in assay buffer for one hour. This is followed by addition of the primary antibody. Finally, the plate is treated with an HRP-labeled secondary antibody and addition of the HRP substrate to produce chemiluminescence signal proportional to the EZH2 activity.

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