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測量應(yīng)用案例-20190904

來源:美國布魯克海文儀器公司   2019年09月18日 09:54  

文獻名: Luminescent carbon nanodots based aptasensors for rapid detection of kanamycin residue

 

作者: Xuexia Lina, Jianlong Sua, Honggui Linb, Xiangying Sunc, Bin Liuc, Ranjith Kumar Kankalaa, Shu-Feng Zhoua

aDepartment of Chemical Engineering& Pharmaceutical Engineering, College of Chemical Engineering, Huaqiao University, Xiamen, 361021, China

bSchool of Marine Engineering, Jimei University, Xiamen, 361021, China

cCollege of Materials Science and Engineering, Huaqiao University, Xiamen, 361021, China

 

摘要:Despite the success in long-term storage of food and dietary products using antibiotics as supplements, enormous levels of their residues have remained as a significant health concern, leading to severe toxicity issues on consumption. Herein, we report an ultrasensitive and highly selective aptasensor based on carbon nanoparticles (CNPs) through a fluorescence-based aptamer-linked immunosorbent assay (FALIA) for rapid detection of kanamycin (KAA) residue. The fabricated CNP-aptasensor exhibited superior selectivity with exceptional photoluminescence properties. Under the optimal conditions, the linear equation of standard KAA solution was Y = −0.2279LogX+1.3648 (R = −0.9893) ranged from 10−4 to 10−7?ppb with excellent relative standard deviations (RSD) between 3.12 and 5.59 % (n?=?3). Moreover, the limit of detection (LOD) was lower than 5.0?×?10−8?ppb. Together, the excellent recovery and significant efficacy in the rapid detection of antibiotics at a low level in milk indicate that this fabricated CNP-aptasensor has a great potential in the establishment of an efficient antibiotic detector system in food and other nutraceutical industries.

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