BGC-823-LUC人低分化前胃癌細(xì)胞-熒光素酶標(biāo)記 含STR

細(xì)胞英文(簡(jiǎn)稱(chēng)）：BGC-823、BGC823

細(xì)胞名稱(chēng)：BGC-823、BGC823人胃癌細(xì)胞【已通過(guò)STR鑒定】

背景資料

該細(xì)胞源自一位62歲的胃癌（未分化腺癌）患者，表達(dá)CEA。 

細(xì)胞來(lái)源：ATCC

代次：P3

規(guī)格：T25

細(xì)胞數(shù)：1x10^6 cells

組織來(lái)源：胃癌

BGC-823、BGC823人胃癌細(xì)胞【已通過(guò)STR鑒定】形態(tài)：貼壁；上皮細(xì)胞樣

細(xì)胞活力：95％（Viability by Trypan Blue Exclusion）

細(xì)胞檢測(cè)：細(xì)胞不含有HIV-1、HBV、HCV、支原體、細(xì)菌、酵母和真菌

培養(yǎng)條件：RPMI-1640 +10% FBS；37℃，5% CO2

傳代方法：*建議1:2-1:3 兩天換液一次

凍存條件：90%FBS+10%DMSO

動(dòng)物細(xì)胞培養(yǎng),大致的步驟是這樣:

1.從動(dòng)物胚胎或者一些剛出生的動(dòng)物的器官或者組織上取得細(xì)胞,配制成細(xì)胞懸浮液.把細(xì)胞液放到培養(yǎng)瓶中,在培養(yǎng)箱里培養(yǎng)--------原代培養(yǎng).

但是,不要以為這樣就能一直無(wú)限培養(yǎng)下去.

因?yàn)锽GC-823、BGC823人胃癌細(xì)胞【已通過(guò)STR鑒定】在那個(gè)瓶壁上生長(zhǎng)的時(shí)候,如果大量增殖,細(xì)胞之間會(huì)彼此相碰,細(xì)胞細(xì)胞就會(huì)停止分裂增殖,出現(xiàn)一種接觸抑制.

2.我們?yōu)榱怂鼈兡芾^續(xù)增殖,就用胰蛋白酶再把它們分開(kāi),然后再配制成細(xì)胞懸浮液,分開(kāi)裝到好幾個(gè)瓶子里繼續(xù)培養(yǎng)--------傳代培養(yǎng).

細(xì)胞株&細(xì)胞系

1.它們都是傳代培養(yǎng)里面的概念.都是10代以后的

2.細(xì)胞株是傳代培養(yǎng)里10~50（不同物種不同組織細(xì)胞有差別吧）代,細(xì)胞系是50代以后的;

3.對(duì)于細(xì)胞繁殖而言,傳到10代就很不容易了,所以細(xì)胞株是極少數(shù)的細(xì)胞

4.50代以后,細(xì)胞繁殖會(huì)出現(xiàn)另一個(gè)危機(jī),基本上沒(méi)有什么細(xì)胞能再傳下去了.但是,有細(xì)胞發(fā)生了基因突變,像一個(gè)癌細(xì)胞一樣無(wú)限地分裂下去,這就是細(xì)胞系.

BGC-823-LUC人低分化前胃癌細(xì)胞-熒光素酶標(biāo)記 含STR-ATCC保藏中心
【Organism物種來(lái)源】Animal 
【Tissue組織來(lái)源】 
【Cell Type細(xì)胞類(lèi)型】 
【Product Format產(chǎn)品狀態(tài)】 frozen/live culture
【Morphology細(xì)胞形態(tài)】 
【Culture Properties細(xì)胞特性】 
【Biosafety Level生物安全級(jí)別】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

【Disease細(xì)胞源疾病】
【Age年齡】 
【Gender性別】 Male/Female
【Storage Conditions保存條件】 liquid nitrogen vapor phase
【Karyotype染色體組型】 
【Images細(xì)胞圖片】 Cell Micrograph
【Derivation衍生細(xì)胞】
【Clinical Data臨床數(shù)據(jù)】 
【Comments其他描述】 
【Complete Growth Medium*培養(yǎng)基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. 
【Subculturing傳代方法】 
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation凍存條件】 
【Freeze Medium凍存培養(yǎng)基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存溫度】 Liquid nitrogen vapor phase
【Culture Conditions培養(yǎng)條件】 
【Atmosphere培養(yǎng)環(huán)境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培養(yǎng)溫度】 37°C
【STR Profile圖譜】 
【Population Doubling Time倍增殖時(shí)間】
【References參考文獻(xiàn)】

更多標(biāo)記細(xì)胞如下：