有絲分裂測定法裝配 有絲分裂紡錘體病癥量化檢測分析工具

Mitotic assembly assays

Quantification of mitotic spindle disorders

READ-OUTS

Spindle assembly, chromosome segregation, flat spindle, spindle robustness, spindle dynamics, fine analysis of microtubules, centrosomes, kinetochores and interaction of other organelles .

STANDARD CULTURE LIMITATION

The mitotic spindle is a compact 3D structure assembling in a very short time with many redundant mechanisms which makes it tedious to analyze, especially its subtle changes.

MITOTIC ASSEMBLY ASSAYS

In this assay cells are observed while compressed in between two parallel surfaces, which gives them a flat shape. In this configuration, events such as spindle assembly, spindle robustness, interactions between organelles can be easily observed. In addition, the mechanical stress imposed on the spindle make it easier to destabilize allowing measurements of subtle changes in its mechanisms of assembly.

EXAMPLE

Effect of confinement in mitosis of HeLa cells [1].

REFERENCES

[1] Lancaster, O. et al.(2013). Developmental Cell, 25(3), 270–283.

[2] Cadart, C. et al.(2014). Developmental Cell, 29(2), 159–169.

提供實現(xiàn)工具Cellconfiner：

Cell confinement

 細胞被均勻地限制/壓縮在兩個亞微米分辨率的兩個平行表面之間。不同的限制高度（例如1um – 300um），允許長期細胞培養(yǎng)和細胞增殖，

同時保持對封閉的控制

與高分辨率光學(xué)顯微鏡系統(tǒng)兼容，

可以處理足夠多的細胞以進行完整的基因表達分析，

可與生物功能化的微結(jié)構(gòu)化底物和/或不同的基質(zhì)（幾何形狀控制）結(jié)合使用
可以與凝膠結(jié)合（硬度控制），

兼容任何細胞培養(yǎng)底物（培養(yǎng)皿至96孔板）