READ-OUTS

Beating frequency, observation of sarcomeres

STANDARD CULTURE LIMITATION

In standard cultures, cardiomyocytes derived from pluripotent stem cells have random shapes, are not organized thus showing immature properties.

CARDIOMYOCYTE MATURATION ASSAY

When cultured in a substrate with line adhesive cues, cardiomyocytes acquire elongated shapes, which regulates cell contractility and establishes and maintains myofibril alignment and an improved and regular cell beating.

EXAMPLE

Organization of cardiomyocytes in line patterns [1], and effect of myofibril alignment in contractility forces.

REFERENCES

[1] hPSC-CM non patterned (left image) and on line micropatterns (right image) imaged by fluorescence microscopy. Dussaud S., Jouve C., Hulot J.S., 2018. These images were obtained through the courtesy of Professor Hulot (Hôpital Européen Georges-Pompidou) and his team, using 4Dcell products.

[2] Max R. Salick, Wendy C. Crone. et al. Micropattern Width Dependent Sarcomere Development in Human ESC-Derived Cardiomyocytes, Biomaterials. 2014, May. 35(15): 4454-4464.

4DCELL DEVICE

Micropatterns

READ-OUTS

Beating frequency, observation of sarcomeres

STANDARD CULTURE LIMITATION

In standard cultures, cardiomyocytes derived from pluripotent stem cells have random shapes, are not organized thus showing immature properties.

CARDIOMYOCYTE MATURATION ASSAY

When cultured in a substrate with line adhesive cues, cardiomyocytes acquire elongated shapes, which regulates cell contractility and establishes and maintains myofibril alignment and an improved and regular cell beating.

EXAMPLE

Organization of cardiomyocytes in line patterns [1], and effect of myofibril alignment in contractility forces.

REFERENCES

[1] hPSC-CM non patterned (left image) and on line micropatterns (right image) imaged by fluorescence microscopy. Dussaud S., Jouve C., Hulot J.S., 2018. These images were obtained through the courtesy of Professor Hulot (Hôpital Européen Georges-Pompidou) and his team, using 4Dcell products.

[2] Max R. Salick, Wendy C. Crone. et al. Micropattern Width Dependent Sarcomere Development in Human ESC-Derived Cardiomyocytes, Biomaterials. 2014, May. 35(15): 4454-4464.