国产精品视频一区二区三区四,亚洲av美洲av综合av,99国内精品久久久久久久,欧美电影一区二区三区电影

產(chǎn)品推薦:氣相|液相|光譜|質(zhì)譜|電化學(xué)|元素分析|水分測定儀|樣品前處理|試驗機|培養(yǎng)箱


化工儀器網(wǎng)>技術(shù)中心>專業(yè)論文>正文

歡迎聯(lián)系我

有什么可以幫您? 在線咨詢

恒遠產(chǎn)品文獻:多聚甲醛試劑引用文獻

來源:上海恒遠生物科技有限公司   2021年01月11日 15:10  
【文獻標(biāo)題】Human umbilical cord mesenchymal stem cell-derived exosomes suppress dermal fibroblasts-myofibroblats transition via inhibiting the TGF-β1/Smad 2/3 signaling pathway
【作者】Jian Hu, Yuanwen Chen, Yubin Huang,et.al
【作者單位】深圳市寶安區(qū)人民醫(yī)院(The People’s Hospital of Bao’an Shenzhen)
【文獻中引用產(chǎn)品】
多聚甲醛
【關(guān)鍵詞】Human umbilical cord mesenchymal stem cells,Exosome, TGF-β1/Smad2/3 signaling pathway,Dermal ibroblasts Myofibroblast
【DOI】/doi.org/10.1016/j.yexmp.2020.104468
【影響因子(IF)】2.39
出版期刊】《Experimental and Molecular Pathology》
【產(chǎn)品原文引用】
The hUC-MSCs or fibroblasts in exponential phase were seeded at 1 × 105 cells/mL and 3 mL/well in a 6-well plate with a cover glass. After culture for 24 h at 37℃ with 5% CO2, culture medium was discarded, and cells were fixed with paraformaldehyde (Shanghai HengYuan 
Biological Technology Co., Ltd., Shanghai, China) for 10 min. After discarding the fixative solution, the cover glasses were rinsed by PBS 3 times, each for 3 min. PBS was then absorbed with absorbent papers, normal goat serum was dripped on the glass, and fibroblasts were sealed for 30min at room temperature. The sealing liquid was absorbed by absorbent papers without washes. After that, diluted primary antibodies (supplementary Table 1) were dripped into each cover glass, which was incubated in a wet box at 4℃ overnight. Afterwards, the fluorescent secondary antibody goat anti-rat IgG H&L (Alexa Fluor® 488) (1/200, ab150113) was added. Then the glass was dripped with 4',6-diamidino-2-phenylindole (DAPI) for nucleus staining, and sealed with sealing liquid containing anti-fluorescent quenching agent, and then observed under the fluorescence microscope. The above antibodies were all purchased from Abcam Inc. (Cambridge, MA, USA).
完整版PDF文獻請咨詢在線客服或者聯(lián)系我司業(yè)務(wù)員!
更多公司福利請關(guān)注“恒遠生物”VX公眾號!

 

免責(zé)聲明

  • 凡本網(wǎng)注明“來源:化工儀器網(wǎng)”的所有作品,均為浙江興旺寶明通網(wǎng)絡(luò)有限公司-化工儀器網(wǎng)合法擁有版權(quán)或有權(quán)使用的作品,未經(jīng)本網(wǎng)授權(quán)不得轉(zhuǎn)載、摘編或利用其它方式使用上述作品。已經(jīng)本網(wǎng)授權(quán)使用作品的,應(yīng)在授權(quán)范圍內(nèi)使用,并注明“來源:化工儀器網(wǎng)”。違反上述聲明者,本網(wǎng)將追究其相關(guān)法律責(zé)任。
  • 本網(wǎng)轉(zhuǎn)載并注明自其他來源(非化工儀器網(wǎng))的作品,目的在于傳遞更多信息,并不代表本網(wǎng)贊同其觀點和對其真實性負責(zé),不承擔(dān)此類作品侵權(quán)行為的直接責(zé)任及連帶責(zé)任。其他媒體、網(wǎng)站或個人從本網(wǎng)轉(zhuǎn)載時,必須保留本網(wǎng)注明的作品第一來源,并自負版權(quán)等法律責(zé)任。
  • 如涉及作品內(nèi)容、版權(quán)等問題,請在作品發(fā)表之日起一周內(nèi)與本網(wǎng)聯(lián)系,否則視為放棄相關(guān)權(quán)利。
企業(yè)未開通此功能
詳詢客服 : 0571-87858618
遂溪县| 广西| 康保县| 禹城市| 昌邑市| 惠水县| 建宁县| 沙雅县| 虎林市| 孟连| 神农架林区| 贡觉县| 光山县| 逊克县| 丰顺县| 河北省| 上杭县| 栾城县| 长兴县| 靖安县| 鄄城县| 嵊州市| 桦川县| 武清区| 霍城县| 工布江达县| 马龙县| 民丰县| 额敏县| 凤凰县| 盱眙县| 黑河市| 海城市| 阿克苏市| 兴仁县| 洮南市| 英吉沙县| 自贡市| 普陀区| 灵武市| 黑龙江省|