国产精品视频一区二区三区四,亚洲av美洲av综合av,99国内精品久久久久久久,欧美电影一区二区三区电影

產(chǎn)品推薦:氣相|液相|光譜|質(zhì)譜|電化學(xué)|元素分析|水分測定儀|樣品前處理|試驗機|培養(yǎng)箱


化工儀器網(wǎng)>技術(shù)中心>專業(yè)論文>正文

歡迎聯(lián)系我

有什么可以幫您? 在線咨詢

技術(shù)文獻:倉鼠褪黑素(MT)ELISA試劑盒引用文獻

來源:上海恒遠生物科技有限公司   2021年01月15日 10:35  

文獻題目  Photoperiod affects Harderian gland morphology and secretion in female Cricetulus barabensis: Autophagy,apoptosis, and mitochondria

作者  Zhe Wang, Jin-Hui Xu, Jun-Jie Mou,et al.

作者單位  曲阜師范大學(xué)

文獻中引用的產(chǎn)品  倉鼠褪黑素(MT)ELISA試劑盒

關(guān)鍵詞  photoperiod, Harderian gland, autophagy, apoptosis, mitochondria

DOI】  doi.org/10.3389/fphys.2020.00408

影響因子(IF)】  3.22

出版期刊   《Frontiers in Physiology》

產(chǎn)品原文引用

Sample Preparation

Hamsters were sacrificed by CO2 asphyxiation at 22:00 on the last day, after all hamsters had been in darkness for at least 2 h. Blood samples were collected immediately from the heart after sacrifice,stored at 4?C for 30 min, and then centrifuged at 3000 rpm for 15 min at 4?C. Serum MT levels were estimated using an enzyme-linked immunosorbent assay (Shanghai Hengyuan Biological Technology Co., Ltd., H-40277, Shanghai, China). The HGs were removed, with lengths and weights then recorded. The HGs from five hamsters were fixed by immersion and processed for light and electron microscopy. The right HGs were immersed in 10% buffered formalin for paraffin section embedding, and the left HGs were immersed in glutaraldehyde-paraformaldehyde for transmission electron microscopy (TEM). The remaining HGs from 15 hamsters in each group were frozen in liquid nitrogen and stored in a refrigerator at −80℃ for subsequent Western blotting, real-time polymerase chain reaction (RTPCR), and immunofluorescence histochemical analyses. In each experiment, unilateral HGs from 10 hamsters were used for quantification. All procedures were carried out in accordance with approved guidelines.

完整版PDF文獻請咨詢在線客服或者聯(lián)系我司業(yè)務(wù)員!
更多公司福利請關(guān)注“恒遠生物”VX公眾號!

免責聲明

  • 凡本網(wǎng)注明“來源:化工儀器網(wǎng)”的所有作品,均為浙江興旺寶明通網(wǎng)絡(luò)有限公司-化工儀器網(wǎng)合法擁有版權(quán)或有權(quán)使用的作品,未經(jīng)本網(wǎng)授權(quán)不得轉(zhuǎn)載、摘編或利用其它方式使用上述作品。已經(jīng)本網(wǎng)授權(quán)使用作品的,應(yīng)在授權(quán)范圍內(nèi)使用,并注明“來源:化工儀器網(wǎng)”。違反上述聲明者,本網(wǎng)將追究其相關(guān)法律責任。
  • 本網(wǎng)轉(zhuǎn)載并注明自其他來源(非化工儀器網(wǎng))的作品,目的在于傳遞更多信息,并不代表本網(wǎng)贊同其觀點和對其真實性負責,不承擔此類作品侵權(quán)行為的直接責任及連帶責任。其他媒體、網(wǎng)站或個人從本網(wǎng)轉(zhuǎn)載時,必須保留本網(wǎng)注明的作品第一來源,并自負版權(quán)等法律責任。
  • 如涉及作品內(nèi)容、版權(quán)等問題,請在作品發(fā)表之日起一周內(nèi)與本網(wǎng)聯(lián)系,否則視為放棄相關(guān)權(quán)利。
企業(yè)未開通此功能
詳詢客服 : 0571-87858618
宾阳县| 万盛区| 东光县| 荃湾区| 汝南县| 上饶县| 平顺县| 新安县| 福海县| 无为县| 乌什县| 通榆县| 沙洋县| 姚安县| 渑池县| 江安县| 南华县| 扎赉特旗| 确山县| 米易县| 马尔康县| 亚东县| 梓潼县| 方城县| 确山县| 麻城市| 丽水市| 张家港市| 政和县| 芜湖市| 封丘县| 日土县| 泸水县| 沙坪坝区| 华安县| 瓦房店市| 宕昌县| 公安县| 屏东市| 堆龙德庆县| 彰武县|