慶大霉素(Gen)ELISA快速檢測(cè)試劑盒（檢組織/肝臟/乳制品，靈敏度0.05ppb，一步法）

慶大霉素(Gen)ELISA快速檢測(cè)試劑盒

一、原理

本試劑盒采用間接競(jìng)爭(zhēng)ELISA方法，在微孔條上預(yù)包被偶聯(lián)抗原，樣本中的殘留物慶大霉素將和微孔條上預(yù)包被的偶聯(lián)抗原競(jìng)爭(zhēng)抗慶大霉素抗體，加入酶標(biāo)記物后，用TMB底物顯色，樣本吸光值與其所含殘留物慶大霉素的含量成負(fù)相關(guān)，與標(biāo)準(zhǔn)曲線比較即可得出相應(yīng)殘留物慶大霉素的含量。

二、試劑盒特性

u  試劑盒靈敏度：       0.05ppb

u  孵育溫度    ：       25℃

u  孵育時(shí)間    ：       30min～15min

u  批內(nèi)、批間差：       <15%

u  樣本檢測(cè)下限

組織  ·····································  2.5 ppb

肝臟  ·····································  3 ppb

牛奶  ·····································  3 ppb

u  交叉反應(yīng)率

慶大霉素   ······································    100%

鏈霉素       ·······································    <1%

雙氫鏈霉素 ········································· <1%

新霉素       ···········································<1%

u  樣本回收率

組織    ·········································  90%±20%

肝臟    ·········································  80%±20%

牛奶  ···········································   90%±18%

三、試劑盒組成

四、樣品前處理步驟及檢測(cè)分析步驟

  詳見(jiàn)產(chǎn)品說(shuō)明書。

五、儲(chǔ)藏條件和保質(zhì)期

1、 儲(chǔ)藏條件：試劑盒于2～8℃保存，不要冷凍。

2、 保 質(zhì) 期：該產(chǎn)品有效期為1年，生產(chǎn)日期見(jiàn)包裝盒。

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