組蛋白是真核生物中負(fù)責(zé)染色體纖維核小體結(jié)構(gòu)的基本核蛋白。這種結(jié)構(gòu)由約146 bp的DNA包裹在核小體周圍，核小體是由四種核心組蛋白（H2A、H2B、H3和H4）中的每一種組成的八聚體。染色質(zhì)纖維通過連接組蛋白H1與核小體之間的DNA相互作用進(jìn)一步緊密，形成高階染色質(zhì)結(jié)構(gòu)。該基因無內(nèi)含子，編碼組蛋白H3家族的一個(gè)成員。該基因的轉(zhuǎn)錄本缺乏多核苷酸序列；相反，它們包含回文終止元素。

該基因位于染色體1上的組蛋白簇中。該基因是集群中四個(gè)重復(fù)的組蛋白基因之一；該記錄代表端粒拷貝。抗組蛋白H3是對(duì)染色質(zhì)修飾劑、染色質(zhì)研究、組蛋白和修飾組蛋白以及表觀遺傳學(xué)研究感興趣的研究人員的理想選擇。

免疫印跡和免疫熒光檢測(cè)抗組蛋白H3[單甲基Lys4]抗體。這種抗體在免疫細(xì)胞化學(xué)、染色質(zhì)免疫沉淀和斑點(diǎn)雜交中很有用。用戶應(yīng)優(yōu)化反應(yīng)性的具體條件。通過HeLa組蛋白制備裂解液或適當(dāng)?shù)募?xì)胞裂解液或提取物中的Western Blotting，預(yù)期約15.4 kDa的條帶對(duì)應(yīng)于組蛋白H3蛋白。Epi Plus™ 與Novus Biologicals合作生產(chǎn)抗體。

Rockland艾美捷組蛋白PTM抗體系列可識(shí)別經(jīng)過修飾的組蛋白和相關(guān)的細(xì)胞靶點(diǎn)，并通過多種檢測(cè)方法進(jìn)行驗(yàn)證。Epi-Plus抗體對(duì)這種修飾是特別靈敏和特異的，并且對(duì)相同位點(diǎn)或該蛋白其他氨基酸的其他修飾沒有交叉反應(yīng)。免疫熒光（IF）、免疫沉淀（IP）、染色質(zhì)免疫沉淀（ChIP）、免疫組化（IHC）和蛋白印跡（WB）的分析驗(yàn)證了Epi-Plus抗體的有效性。

Rockland艾美捷組蛋白PTM抗體（部分推薦）：

600-401-I69 Histone H3 K9me1 Antibody 50ug Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I70 Histone H3 K9me2 Antibody 50ug Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I71 Histone H3 K9me3 Antibody 50ug Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I72 Histone H3 K9ac Antibody 50ug Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I73 Histone H3 K9ac/K14ac Antibody 50ug Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I74 Histone H3 pS10 Antibody 50ug Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I75 Histone H3 pS10/pT11 Antibody 50ug Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I76 Histone H3 pT11 Antibody 50ug Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I77 Histone H3 R17me2a Antibody 50ug Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I78 Histone H3 K18me1 Antibody 50ug Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I79 Histone H3 K18me2 Antibody 50ug Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I80 Histone H3 K18me3 Antibody 50ug Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I81 Histone H3 K18ac Antibody 50ug Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I82 Histone H3 K23ac Antibody 50ug Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant