PureCube His Affinity Agarose (NTA)是針對(duì)組氨酸標(biāo)簽的親和層析產(chǎn)品，組氨酸標(biāo)簽是應(yīng)用廣泛的親和層析標(biāo)簽，這種標(biāo)簽具有小尺寸、低免疫原性的特點(diǎn)和在多種天然或變性條件下的廣泛適用性，NTA相對(duì)于IDA具有更高的洗脫液純度。

產(chǎn)品優(yōu)勢(shì)：

1）德國(guó)制造，品質(zhì)與信譽(yù)的堅(jiān)實(shí)保證；

2）結(jié)合載量可高達(dá)80mg/mL，目前市面上高結(jié)合能力；

3）更*的DTT和EDTA穩(wěn)定性和耐受性；

4）凝膠的多孔性和均勻粒徑，使蛋白與凝膠之間有很好地相互作用；

5）適合低壓環(huán)境下的純化過程（大規(guī)模純化制備或預(yù)裝柱均可使用）；

6）高性價(jià)比，為研發(fā)、生產(chǎn)降低成本；

7）適用于大腸桿菌和真核細(xì)胞的裂解液和上清液；

8）同時(shí)可裝載Co, Cu, Zn和其它金屬離子。

優(yōu)勢(shì)詳解：

1. 收率高，純度高

Fig. 1: Over 20% more yield obtained with PureCube Ni-NTA Agarose. SDS-PAGE of GFP expressed in E. coli and purified in gravity columns with PureCube Ni-NTA Agarose and Ni-NTA resin from Competitor Q. 80 mg/mL protein yield was obtained with PureCube Ni-NTA Agarose (E1–E4, Cube) compared to 65 and 48 mg/mL, respectively, with the widely used alternative resins G and Q (E1–E4, Competitor G / Competitor Q).

2. *的DTT和EDTA穩(wěn)定性

Fig. 2: NTA is robust in the presence of reducing and chelating agents. GFP-His was purified on gravity columns containing PureCube Ni-NTA Agarose after exposing the resin for 1 h to 3 concentrations of DTT or EDTA. NTA exhibits a shallow decay rate in binding capacity.

PureCube Ni-NTA瓊脂糖在DTT和EDTA存在下非常堅(jiān)實(shí)，在穩(wěn)定性測(cè)試中，將PureCube Ni-NTA瓊脂糖置于DTT與EDTA濃度逐漸升高的環(huán)境中處理1小時(shí)。再用處理過的凝膠在重力層析柱中純化大腸桿菌表達(dá)的GFP-His，凝膠的蛋白結(jié)合能力因存在DTT與EDTA而下降，但是衰減率平緩，即使在1.5 mM EDTA，凝膠依然具有其結(jié)合能力的54%。

產(chǎn)品特性：

訂貨信息：

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