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InVivoPlus anti-mouse Ly6G產品介紹

來源:深圳欣博盛生物科技有限公司   2023年04月11日 14:39  

產品介紹

BioXcell InVivoPlus anti-mouse Ly6G 1A8單克隆抗體與小鼠Ly6G反應。Ly6G分子量為21-25kDa,是GPI錨定的細胞表面蛋白Ly-6超家族的成員,在細胞信號傳導和細胞粘附中發(fā)揮作用。Ly6G在發(fā)育過程中由骨髓譜系中的細胞(包括單核細胞、巨噬細胞、粒細胞和嗜中性粒細胞)差異表達。單核細胞通常在發(fā)育過程中瞬時表達Ly6G,而成熟的粒細胞和外周嗜中性粒細胞保持表達,使Ly6G成為這些群體的良好細胞表面標志物。與BioXcell RB6-8C5抗體不同,1A8抗體與小鼠Ly6G特異性反應,而與Ly6C沒有交叉反應性的報道。


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BioXcell熱銷產品推薦--InVivoPlus anti-mouse Ly6G

產品詳情

產品名稱

InVivoPlus anti-mouse Ly6G

產品貨號

BP0075-1

產品規(guī)格

5/25/50/100mg

反應種屬

Mouse

克隆號

1A8

同種型

Rat IgG2a, κ

免疫原

EL4J cells transfected with Ly6G

實驗應用

in vivo neutrophil depletion

in vivo MDSC depletion

Immunofluorescence

Immunohistochemistry (paraffin)

Immunohistochemistry (frozen)

Flow cytometry

產品形式

PBS, pH 7.0,Contains no stabilizers or preservatives

純度

>95%, Determined by SDS-PAGE

聚合

<5%, Determined by SEC

無菌處理

0.2 µm filtration

純化方式

Protein G

RRID

AB_1107721

分子量

150 kDa

小鼠病原檢測

Ectromelia/Mousepox Virus: Negative

Hantavirus: Negative

K Virus: Negative

Lactate Dehydrogenase-Elevating Virus: Negative

Lymphocytic Choriomeningitis virus: Negative

Mouse Adenovirus: Negative

Mouse Cytomegalovirus: Negative

Mouse Hepatitis Virus: Negative

Mouse Minute Virus: Negative

Mouse Norovirus: Negative

Mouse Parvovirus: Negative

Mouse Rotavirus: Negative

Mycoplasma Pulmonis: Negative

Pneumonia Virus of Mice: Negative

Polyoma Virus: Negative

Reovirus Screen: Negative

Sendai Virus: Negative

Theiler’s Murine Encephalomyelitis: Negative

保存條件

抗體原液保存在4°C,不能冷凍保存。

推薦同型對照

InVivoPlus rat IgG2a isotype control, anti-trinitrophenol(貨號BP0089

推薦抗體稀釋液

InVivoPure pH 7.0 Dilution Buffer(貨號IP0070


為什么選擇InVivoPlus抗體?

InVivoPlus級別的產品內毒素含量更低,經過多種實驗驗證,更適合用于體內實驗研究

BioXcell熱銷產品推薦--InVivoPlus anti-mouse Ly6G

該產品自上市已被多篇SCI文獻引用,品質有保證,以下是部分已發(fā)表的文獻引用:


應用

文章

體內中性粒細胞耗竭

(in vivo neutrophil depletion)

1. Davis, R. W. t., et al. (2018). 'Luminol Chemiluminescence Reports Photodynamic Therapy-Generated Neutrophil Activity In Vivo and Serves as a Biomarker of Therapeutic Efficacy' Photochem Photobiol .

2. Moynihan, K. D., et al. (2016). 'Eradication of large established tumors in mice by combination immunotherapy that engages innate and adaptive immune responses' Nat Med. doi : 10.1038/nm.4200.

3. Conde, P., et al. (2015). 'DC-SIGN(+) Macrophages Control the Induction of Transplantation Tolerance' Immunity 42(6): 1143-1158.

4. Griseri, T., et al. (2015). 'Granulocyte Macrophage Colony-Stimulating Factor-Activated Eosinophils Promote Interleukin-23 Driven Chronic Colitis' Immunity 43(1): 187-199.

5. Yamada, D. H., et al. (2015). 'Suppression of Fcgamma-receptor-mediated antibody effector function during persistent viral infection' Immunity 42(2): 379-390.

6. Ellis, G. T., et al. (2015). 'TRAIL+ monocytes and monocyte-related cells cause lung damage and thereby increase susceptibility to influenza-Streptococcus pneumoniae coinfection' EMBO Rep 16(9): 1203-1218.

7. Deshmukh, H. S., et al. (2014). 'The microbiota regulates neutrophil homeostasis and host resistance to Escherichia coli K1 sepsis in neonatal mice' Nat Med 20(5): 524-530.

體內中性粒細胞耗竭、流式細胞術、免疫組化石蠟切片(in vivo neutrophil depletion, Flow Cytometry, Immunohistochemistry (paraffin))

Coffelt, S. B., et al. (2015). 'IL-17-producing gammadelta T cells and neutrophils conspire to promote breast cancer metastasis' Nature 522(7556): 345-348.

體內中性粒細胞耗竭、流式細胞術、免疫組化石蠟切片、免疫組化冰凍切片(in vivo neutrophil depletion, Flow Cytometry, Immunohistochemistry (paraffin), Immunohistochemistry (frozen))

Finisguerra, V., et al. (2015). 'MET is required for the recruitment of anti-tumoural neutrophils' Nature 522(7556): 349-353.

體內中性粒細胞耗竭、流式細胞術(in vivo neutrophil depletion, Flow Cytometry)

1.Moser, E. K., et al. (2014). 'Late engagement of CD86 after influenza virus clearance promotes recovery in a FoxP3+ regulatory T cell dependent manner' PLoS Pathog 10(8): e1004315.

2. Chen, K. W., et al. (2014). 'The neutrophil NLRC4 inflammasome selectively promotes IL-1beta maturation without pyroptosis during acute Salmonella challenge' Cell Rep 8(2): 570-582.

3. Garraud, K., et al. (2012). 'Differential role of the interleukin-17 axis and neutrophils in resolution of inhalational anthrax' Infect Immun 80(1): 131-142.

4. Lee, W. B., et al. (2012). 'Neutrophils Promote Mycobacterial Trehalose Dimycolate-Induced Lung Inflammation via the Mincle Pathway' PLoS Pathog 8(4): e1002614.

體內骨髓來源抑制細胞耗竭(in vivo MDSC depletion)

Deng, L., et al. (2014). 'Irradiation and anti-PD-L1 treatment synergistically promote antitumor immunity in mice' J Clin Invest 124(2): 687-695.

體內中性粒細胞耗竭、免疫熒光(in vivo neutrophil depletion,Immunofluorescence)

Edelson, B. T., et al. (2011). 'CD8alpha(+) dendritic cells are an obligate cellular entry point for productive infection by Listeria monocytogenes' Immunity 35(2): 236-248.





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