Gelatin Flora Buffer (GVB) and Vernal Buffered Saline (VBS) are essential buffers for complement research, while Flora Buffered Saline (VBS) lacks gelatin components compared to Gelatin Flora Buffer (GVB).

Aimijie Gelatin Flora Buffer with Ca++and Mg++:

Cat B102-250mL;  B100-1L

Name GVB++(with Ca++and Mg++) Gelatin Flora Buffer Containing Ca++and Mg++

Ingredients: 0.1% gelatin, 5mM Vernal, 145mM NaCl, 0.025% NaN3, pH7.3 Containing 0.15mM calcium chloride and 0.5mM magnesium chloride

The application should use GVB++to prepare antibody sensitized sheep red blood cells (EA) and clean them before use for testing; GVB++can be used to dilute all components of complement classical pathway and lectin pathway experiments.

Concept:

During the dissolution process, two types of changes occur: one is the diffusion of solute molecules (or ions) into water under the action of water molecules, which absorbs heat; the other is the interaction of solute molecules (or ions) with water molecules to generate water and molecules (or water and ions), which release heat.

There are two changes during the dissolution process of substances:

Diffusion process: The diffusion of solute molecules (or ions) into water is a physical process that absorbs heat

Hydration process: the molecules (or ions) of solute interact with water molecules to generate hydrated molecules (or hydrated ions), which is a chemical process and emits heat

Temperature changes during dissolution process:

a. The heat absorbed during the diffusion process is greater than the heat released during the hydration process, resulting in a decrease in solution temperature, such as NH4NO3 dissolved in water.

b. The heat absorbed during the diffusion process is less than the thermal vibration released during the hydration process, and the solution temperature increases, such as NaOH or concentrated sulfuric acid dissolved in water.

c. The heat absorbed during the diffusion process ≈ the heat released during the hydration process, and the solution temperature remains almost constant, such as NaOl dissolved in water.

Common frozen mixtures:

Ice is commonly used as a coolant in production and daily life, but it can only provide a low temperature of around 0 ℃. Some medical and research institutions often require strict and low temperatures to provide convenient access to low temperatures

The method is to use a frozen mixture.

明膠弗洛拿緩沖液(Gelatin Veronal Buffer, GVB)和弗洛拿緩沖的生理鹽水(Veronal Buffered Saline, VBS)是補(bǔ)體研究的必-備緩沖液, 弗洛拿緩沖的生理鹽水(VBS)和明膠弗洛拿緩沖液(GVB)相比缺少明膠組分。   

艾美捷含Ca++和Mg++的明膠弗洛拿緩沖液：

Cat B102-250mL; B100-1L

名稱 GVB++ (with Ca++ and Mg++)含Ca++和Mg++的明膠弗洛拿緩沖液

成分 0.1% gelatin, 5mM Veronal, 145mM NaCl, 0.025% NaN3 , pH7.3. Containing 0.15mM calcium chloride and 0.5mM magnesium chloride

應(yīng)用 應(yīng)使用GVB++制備抗體致敏羊紅細(xì)胞(EA)，并在用于測定前對其進(jìn)行清洗；GVB++可用于稀釋補(bǔ)體經(jīng)典途徑和凝集素途徑試驗(yàn)的所有成分。

概念：

在溶解過程中發(fā)生了兩種變化，一種是溶質(zhì)的分子（或離子）在水分子的作用下向水中擴(kuò)散，這一過程吸收熱量：另一種是擴(kuò)散的溶質(zhì)的分子（或離子）和水分子作用，生成水和分子（或水和離子），這一過程放出熱量。

物質(zhì)溶解過程中的兩種變化：

擴(kuò)散過程：溶質(zhì)的分子（或離子）向水中擴(kuò)散，是物理過程，吸收熱量

水合過程：溶質(zhì)的分子（或離子）和水分子作用，生成水合分子（或水合離子），是化學(xué)過程，放出熱量

溶解過程中的溫度變化：

a. 擴(kuò)散過程中吸收的熱量>水合過程中放出的熱量，溶液溫度降低，如：NH4NO3溶解于水。

b. 擴(kuò)散過程中吸收的熱量<水合過程中放出的熱振，溶液溫度升高，如：NaOH、濃硫酸溶解于水。

c. 擴(kuò)散過程中吸收的熱量≈水合過程中放出的熱量，溶液溫度幾乎不變，如：NaOl溶解于水。

常見冷凍混合物：

在生產(chǎn)、生活中常用冰作冷卻劑，但冰只能提供，0℃左右的低溫。一些醫(yī)療和研究單位常需要嚴(yán)低的溫度，提供低溫較方便的

方法是用冷凍混合物。

下面是幾種常見冷凍混合物的組成及所能達(dá)到的低溫度。

冷冰混合物的組成 低溫度

1gH4NO3和100g冰

19MH4C1和100g冰 -16

23gNaCl和100g冰 -21

22gMgC12和100g冰 -34