海腎熒光素酶測(cè)定試劑盒2.0是Biotium的海腎熒光素酶測(cè)定試劑盒（目錄號(hào)30004）的改進(jìn)版本，具有簡(jiǎn)化的方案和改進(jìn)的工作溶液穩(wěn)定性。該試劑盒還具有Biotium的水溶性Aquaphile腔腸素。

艾美捷海腎熒光素酶測(cè)定試劑盒2.0,150次測(cè)定#BTM-30082-1組件：

5X 被動(dòng)裂解緩沖液

海腎螢光素酶檢測(cè)緩沖液 2.0

Aquaphile™ 腔腸素

海腎熒光素酶測(cè)定試劑盒相關(guān)特征：

高靈敏度和線性快速型 RLuc 測(cè)定

易于處理的水溶性基材

高性?xún)r(jià)比

海腎熒光素酶測(cè)定試劑盒屬性：

化驗(yàn)類(lèi)型/選項(xiàng)：終點(diǎn)測(cè)定，發(fā)光（閃光型）

檢測(cè)方法/讀數(shù)：光度計(jì)（帶試劑注射器的單管或微量板讀取器）

儲(chǔ)存條件：在-60°C或更低溫度下儲(chǔ)存

Sea kidney Luciferase has been used as a reporter gene to study gene regulation and function in vitro and in vivo. It is usually used for multiple transcriptional reporter gene detection or as a standardized transfection control for firefly Luciferase detection. Renila Luciferase is a 36000 Dalton monomer protein that catalyzes the oxidation of coelenterazine by oxygen to produce light. The activity of this enzyme does not require post translational modification and can serve as a genetic reporter gene immediately after translation. Coelenterazine also emits light through enzyme independent oxidation, a process called self luminescence. Superoxide anions and peroxynitrite in cells and tissues enhance self luminescence. This test kit uses a special buffer formula, which aims to conduct reliable linear measurement of Luciferase activity in the sea and kidney with the minimum self luminous background and excellent sensitivity.

Note: This reagent kit is a flash type luminescence detection that requires immediate measurement of luminescence after adding reagents to your sample. It requires a single tube luminescent meter or a luminescent enzyme-linked immunosorbent assay instrument with a reagent syringe.

Easy to use for consistent results

This kit is a new improved substitute for Haishen Luciferase Test Kit (Catalog No. 30004). In addition to providing a simplified experimental scheme, Renilla Luciferase Test Kit 2.0 also has Biotium Aquaphile ™  Coelenterazine, a water-soluble substrate, is different from coelenterazine's methanol solution. It can be stored at - 20 ° C with the minimum evaporation.

The measurement quantity is based on a 96 well measurement format. This kit contains sufficient passive lysis buffer to perform the specified number of measurements within the plate size range of 96 to 24 wells.