血紅素加氧酶-1 (HO-1)也稱為Hsp32，是血紅素加氧酶的誘導(dǎo)型異構(gòu)體，對血紅素的NADPH、氧和細胞色素P450還原酶依賴性氧化進行催化，氧化為一氧化碳、亞鐵和膽綠素，膽綠素迅速還原為膽紅素。這些反應(yīng)產(chǎn)物具有重要的生理作用：一氧化碳是一種有效的血管擴張劑，并被認為是cGMP和血管緊張度的生理調(diào)節(jié)劑；膽綠素及其產(chǎn)物膽紅素是有效的抗氧化劑；“游離”鐵通過影響鐵調(diào)節(jié)蛋白(IRP)-1的構(gòu)象及其與mRNAs的5’-或3’-UTR中的鐵調(diào)節(jié)元件(IREs)的結(jié)合，增加氧化應(yīng)激并調(diào)節(jié)許多mRNAs(例如，DCT-1、鐵蛋白和轉(zhuǎn)移受體)的表達。三種已鑒定的血紅素加氧酶亞型是催化血紅素轉(zhuǎn)化為膽綠素和一氧化碳的血紅素加氧酶系統(tǒng)的一部分，分別是可誘導(dǎo)的HO-1或Hsp32，在腦和睪丸中大量存在的組成型HO-2，以及與HO-2相關(guān)但為不同基因產(chǎn)物的HO-3。

Enzo Life Sciences的HO-1 (human), ELISA kit提供了一種檢測和定量人源樣品中血紅素氧合酶(HO-1)的方法。該試劑盒可以精確地測定細胞裂解物（cell lysates）和組織提取物（tissue extracts）中的HO-1，不與人HO-2或HO-3(其他已知的血紅素加氧酶同種型)交叉反應(yīng)。

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產(chǎn)品特點

● 高靈敏度，可檢測低至0.78 ng/ml的HO-1

● 重復(fù)可靠的檢測結(jié)果

● 高通量，可在2.5h小時內(nèi)完成多達40個樣本的檢測（含復(fù)孔）

● 優(yōu)于半定量Western blot分析的全定量檢測

產(chǎn)品信息

標(biāo)準曲線示例

部分產(chǎn)品文獻引用

1. Does a negative correlation of heme oxygenase-1 with hematoma thickness in chronic subdural hematomas affect neovascularization and microvascular leakage? A retrospective study with preliminary validation: H.T. Luh, et al.; J. Neurosurg. (2023) 

2. Delivery of self-replicating messenger RNA into the brain for the treatment of ischemic stroke: M. Kim, et al.; J. Control Release 350, 471 (2022) 

3. Effects of Tolvaptan on Oxidative Stress in ADPKD: A Molecular Biological Approach: M. Rigato, et al.; J. Clin. Med. 11, 402 (2022) 

4. Associations between blood heavy metal(loid)s and serum heme oxygenase-1 in pregnant women: Do their distribution patterns matter?: K. Li, et al.; Environ. Pollut. 286, 117249 (2021) 

5. Dual-Functional Dendrimer Micelles with Glycyrrhizic Acid for Anti-Inflammatory Therapy of Acute Lung Injury: M. Choi, et al.; ACS Appl. Mater. Interfaces 13, 47313 (2021) 

6. Induction of HO-1 by 5, 8-Dihydroxy-4,7-Dimethoxyflavone via Activation of ROS/p38 MAPK/Nrf2 Attenuates Thrombin-Induced Connective Tissue Growth Factor Expression in Human Cardiac Fibroblasts: C. Yang, et al.; Oxid. Med. Cell. Longev. 2020, 1080168 (2020) 

7. Beneficial use of serum ferritin and heme oxygenase-1 as biomarkers in adult-onset Still's disease: A multicenter retrospective study: Y. Kirino, et al.; Mod. Rheumatol. 28, 858 (2018), Application(s): ELISA using human serum 

8. Camel Milk Ameliorates 5-Fluorouracil-Induced Renal Injury in Rats: Targeting MAPKs, NF-κB and PI3K/Akt/eNOS Pathways: H.H. Arab, et al.; Cell. Physiol. Biochem. 46, 1628 (2018), Application(s): ELISA with Rat 

9. ELISA Development for Serum Hemeoxygenase-1 and Its Application to Patients with Acute Respiratory Distress Syndrome: Y. Hara, et al.; Can. Respir. J. 2018, 9627420 (2018), Application(s): Human serum 

10. Endothelin-1-induced hypertrophic alterations and heme oxygenase-1 expression in cardiomyoblasts are counteracted by beta estradiol: in vitro and in vivo studies: T. Barta, et al.; Naunyn Schmiedebergs Arch. Pharmacol. 391, 371 (2018) 

11. Haemolysis and haem oxygenase-1 induction during persistent 'asymptomatic' malaria infection in Burkinabé children: J.P. Mooney, et al.; Malar. J. 17, 253 (2018) 

12. Hepatoprotective Potential of Standardized Ficus Species in Intrahepatic Cholestasis Rat Model: Involvement of Nuclear Factor-κB, and Farnesoid X Receptor Signaling Pathways: S.S. El-Hawary, et al.; J. Ethnopharmacol. 231, 262 (2018)

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