SouthernBiotech的明膠底玻片已經(jīng)過仔細(xì)清潔，并用0.1%明膠和硫酸鉀鉻的溶液進行了底涂。涂有涂層的載玻片尺寸為25毫米x 75毫米，帶有磨砂端用于標(biāo)記。為了獲得合適的附著力，SouthernBiotech建議厚度小于100μm的部分。

艾美捷SouthernBiotech丨明膠底玻片（SLD01-CS）的應(yīng)用：

冰凍切片——文獻(xiàn)3,6中報道

自由漂浮冷凍切片——文獻(xiàn)4,5,7,8中報道

樹脂嵌入部分——文獻(xiàn)2中報道

TUNEL——文獻(xiàn)1中報道

圖：自由漂浮Bcs1lc.232A>Gmouse腦皮質(zhì)冷凍切片用抗GFAP染色，然后二次試劑DAB，并安裝在明膠載玻片上（SB目錄號SLD01）。圖片來自Tegelberg S、Toma?id N、Kallj?rvi J、Purhonen

J、 埃爾默

SouthernBiotech丨明膠底玻片參考文獻(xiàn)：

1. Lee T, Zhang X, Dhar S, Faas H, Lippard SJ, Jasanoff A. In vivo imaging with a cell-permeable porphyrin-based MRI contrast agent. Chem Biol.

2010;17:665-73. (TUNEL)

2. Curcio CA, Messinger JD, Sloan KR, Mitra A, McGwin G, Spaide RF. Human chorioretinal layer thicknesses measured in macula-wide, highresolution histologic sections. Invest Ophthalmol Vis Sci. 2011;52:3943-54. (Resin embedded sections)

3. Deane EC, Ilie AE, Sizdahkhani S, Gupta MD, Orlowski J, McKinney RA. Enhanced recruitment of endosomal Na+/H+ exchanger NHE6 into dendritic spines of hippocampal pyramidal neurons during NMDA receptor-dependent long-term potentiation. J Neurosci. 2013;33:595-610. (Frozen sections)

4. Hodor A, Palchykova S, Baracchi F, Noain D, Bassetti CL. Baclofen facilitates sleep, neuroplasticity, and recovery after stroke in rats. Ann Clin Transl Neurol. 2014;1:765-77. (Free floating frozen sections)

5. Kim KY, Scholl ES, Liu X, Shepherd A, Haeseleer F, Lee A. Localization and expression of CaBP1/caldendrin in the mouse brain. Neuroscience.2014;268:33-47. (Free floating frozen sections)

6. Adjei S, Wesson DW. Laminar and spatial localization of the islands of Calleja in mice. Neuroscience. 2015;287:137-43. (Frozen sections)

7. Tegelberg S, Toma?i? N, Kallij?rvi J, Purhonen J, Elmér E, Lindberg E, et al. Respiratory chain complex III deficiency due to mutated BCS1L: a novel phenotype with encephalomyopathy, partially phenocopied in a Bcs1l mutant mouse model. Orphanet J Rare Dis. 2017;12:73. (Free floating frozen sections)

8. Morabito MV, Ravussin Y, Mueller BR, Skowronski AA, Watanabe K, Foo KS, et al. Weight perturbation alters leptin signal transduction in a regionspecific manner throughout the brain. PLoS One. 2017;12(1): e0168226. (Free floating frozen sections)

SouthernBiotech 致力于高質(zhì)量、高親和純化的二抗的生產(chǎn)、純化和標(biāo)記。  同時，SBA還提供各種親和純化的多克隆和單克隆抗體，其中包括：熒光素和酶標(biāo)記物，低內(nèi)毒素/不含疊氮化物（LE/AF）制劑，F(ab)和 F(ab')2片段化抗體，此外還有純化的免疫球蛋白、血清以及細(xì)胞外基質(zhì)蛋白等產(chǎn)品。艾美捷科技是SouthernBiotech的中國代理商，為科研工作者提供優(yōu)質(zhì)的產(chǎn)品與服務(wù)。